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Device for detecting oral lichen planus precancerous lesions

A technology for oral lichen planus and precancerous lesions, applied in the field of biotechnology and diagnosis, can solve problems such as metastasis, incurable cancer, and poor overall prognosis, and achieve the effect of predicting risks

Pending Publication Date: 2020-02-28
赤峰学院附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cancer is almost always diagnosed by a specialist looking at a sample of cells or tissue under a microscope. In some cases, tests on the cells' proteins, DNA, and RNA can help tell doctors whether cancer is present. These test results play an important role in choosing the best treatment options. Important at times, these tests are usually performed when an individual has self-registration of the disorder, and in these cases, if it is cancer, it is likely that the cancer has progressed to the point where simple surgery is not curable or the likelihood of metastasis is significant, leading to the need for chemotherapy and / or radiation and overall poor prognosis
On the other hand, when the condition is not caused by cancer, the patient is left in limbo for a considerable time while waiting for the results of the biopsy

Method used

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  • Device for detecting oral lichen planus precancerous lesions
  • Device for detecting oral lichen planus precancerous lesions
  • Device for detecting oral lichen planus precancerous lesions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A detection device for precancerous lesions of oral lichen planus, including Golli-MPB, STIM1, FGF19, FGFR4 four protein markers and a kit using the four markers as detection targets, according to the four markers The development stage of precancerous lesions of lichen planus can be judged by the expression level of the mRNA of the substance, and the kit includes fluorescent quantitative PCR reagent, Western-blot western blot, reverse transcription reagent and DAB chromogenic reagent.

[0038] The fluorescent quantitative PCR reagent, Western-blot and reverse transcription reagent detect two proteins, Golli-MPB and STIM1, and the DAB chromogenic reagent detects two proteins, FGF19 and FGFR4.

Embodiment 2

[0040] Excluding patients with other oral diseases, autoimmune diseases, taking immune preparations and taking drugs that may cause lichenification, 50 adult patients with OLP were selected as the experimental group, and 50 healthy adults were selected as the control group. Neither was statistically significant.

[0041]Fluorescent quantitative PCR: (1) Extraction of total RNA from the sample: transfer the purified peripheral blood lymphocytes to an enzyme-free and pyrogen-free tube, centrifuge, discard the supernatant, add 1ml Trizol to mix, and let stand on ice for 10 minutes; Chloroform, mix well, let stand on ice for 2-5min, and centrifuge at 12000r / min for 15min at 4°C; Centrifuge at 12000r / min for 10min at 4°C for 10min; discard the supernatant, wash the pellet gently with 75% ethanol, then centrifuge at 12000r / min for 10min at 4°C; discard the supernatant, and dry the RNA pellet in a fume hood 2-3min; after the dried RNA precipitate was dissolved in DEPC water, reverse...

Embodiment 3

[0044] On the basis of Example 2, combined with figure 1 and figure 2 , the results of real-time fluorescence quantification showed that the expression level of Golli-MPB mRNA in the experimental group was significantly higher than that in the normal control group, P<0.001, and the expression level of STIM1 mRNA in the OLP group was significantly higher than that in the normal control group, P<0.001. According to the Western blot results, the expression levels of Golli-MPB and STIM1 proteins in the experimental group were also significantly higher than those in the normal control group.

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Abstract

The invention relates to a device for detecting oral lichen planus precancerous lesions. The device comprises four protein markers including Golli-MPB, STIM1, FGF19 and FGFR4 and a kit using the fourmarkers as the detection targets and is characterized in that the development stage of the oral lichen planus precancerous lesions is judged according to the mRNA expression quantity of the four markers, and the kit comprises a fluorogenic quantitative PCR reagent, Western-blot, a reverse transcription reagent and a DAB chromogenic reagent. By the device, the oral lichen planus precancerous lesionrisk can be predicted effectively.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and diagnosis, and in particular relates to a detection device for oral lichen planus precancerous lesions. Background technique [0002] Oral lichen planus (OLP) is a common oral mucosal disease with unknown etiology, easy to relapse, and has a certain tendency to become cancerous. It is defined as a precancerous state by the World Health Organization. The pathogenesis of oral flat moss is not yet clear. Current research shows that it may be related to immunity, spirit, endocrine, infection, microcirculation disturbance, heredity, systemic disease, and oral local stimulation factors. Among them, the local immune response mediated by cells is disordered. It plays an important role in the occurrence and development of the disease. The clinical use of glucocorticoids and chloroquine and other immunosuppressants is effective, and it has also been proved that the disease is related to immune fac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886G01N33/57484G01N33/57407C12Q2600/118C12Q2600/158C12Q2600/166
Inventor 张沁
Owner 赤峰学院附属医院
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