Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit

A technology of troponin and complexes, which is applied in the field of immunoassay analysis technology and nanobiology, can solve the problems of increase and long duration, achieve long storage time, wide range of standard curve, and improve detection accuracy

Pending Publication Date: 2020-02-25
浙江博实生物科技有限公司
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the half-life of troponin is very short (cTnT 2 hours, free cTnI 2h~5d), its degradation process from myofibrils lasts for a long time, and it can remain elevated in the blood for a long time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit
  • Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit
  • Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail:

[0041] As an embodiment of the double-labeled kit and preparation and detection method for detecting troponin and complexes described in the present invention, the reaction principle is as follows: Figure 1-2 , an antibody against the cTnI region protected by endogenous cTnC serves as a solid-phase antibody to capture all cTnI, an antibody against cTnC is labeled Eu for detection of cTnI-C complexes, and an antibody against the end of cTnI is labeled with Sm , used to detect the complete fragment of cTnI, because the end of cTnI is easily hydrolyzed after entering the blood, so that the complete cTnI is reduced, and the cTnI-C complex exists more stably, so that the complete cTnI and cTnI-C complex can be detected , and calculating its ratio will be helpful in judging the duration of the onset of AMI.

[0042] In this embodiment, the double-labeled t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a double-labeling kit for detecting troponin (Tn) and a compound and preparation and detection methods of the double-labeling kit, and belongs to the field of immunodetectionanalysis technologies and nano-biotechnologies. The kit comprises a reaction buffer solution integrated on a reagent strip, a cleaning solution, an enhancement solution, a magnetic particle solution coating a cTnI monoclonal antibody, a europium-labeled cTnC monoclonal antibody solution and a samarium-labeled cTnI monoclonal antibody solution and 2-6 bottles of additional freeze-dried calibrationproducts of natural cTnI antigens (containing cTnC) with different concentrations. The kit can provide a nearly-homogeneous double-labeling reaction system to ensure that the content of cTnI with complete fragments and the cTnI-C compound is detected simultaneously, the detection time is shortened, the efficiency is improved, and convenience is brought to clinical use.

Description

technical field [0001] The invention belongs to the field of immunoassay analysis technology and nano-biotechnology, in particular to a double-labeled kit for detecting troponin and its complex and a preparation and detection method. Background technique [0002] Cardiac troponin (cTn) is a regulatory protein of cardiac muscle contraction. cTn is composed of subunits of three different genes: cardiac troponin I (cTn I) and troponin C (TnC), and cardiac troponin T (cTnT). [0003] cTn exists in cardiomyocytes in the form of cTnI-C-T complex and free cTnI. After the myocardial injury is released into the blood circulation, cTnI-C-T can be further decomposed into cTnI-C complex and free cTnT, and cTnI-C is its The major form in the blood. Since their content in normal serum is very small, they are significantly increased in acute myocardial infarction (AMI). When cardiomyocytes are damaged by factors such as ischemia and hypoxia, free cTnI and cTnT can be rapidly released fr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/68G01N33/577
Inventor 黄飚任志奇静亚茹
Owner 浙江博实生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com