Molecular marker of low-temperature-sensitive genic male sterile gene and application thereof

A molecular marker, nuclear sterility technology, applied in the field of crop genetics and breeding, can solve the problems of high risk of seed production, impracticality, and difficulty in guaranteeing, to broaden the geographical scope, enrich the types of practical sterile resources, reduce The effect of seed production risk

Inactive Publication Date: 2020-02-07
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these GMS materials, since most of the fertility conversion critical temperatures are between 22 and 25°C (Cai Yidong, 2003), the risk of seed production is extremely high. It is difficult to guarantee that there will be no weather with an average daily temperature higher than 25°C, so it is not practical

Method used

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  • Molecular marker of low-temperature-sensitive genic male sterile gene and application thereof
  • Molecular marker of low-temperature-sensitive genic male sterile gene and application thereof
  • Molecular marker of low-temperature-sensitive genic male sterile gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Genetic Analysis of Yannong S Low Temperature Sensitive Genic Sterile Traits

[0022] Taking Yannong S (YNS) as the female parent and crossing with the fertile parent Samsara 422 (L422), F 1 and their parents were planted in artificial climate chambers at 26°C and 30°C. Under the condition of 26℃, both F1 and L422 were male fertile, but YNS was male sterile, which indicated that the low temperature sensitive sterility trait was recessive.

[0023] YNS / L422F 2 Groups were planted in Lingshui, Hainan (short-day low temperature conditions). Observation and statistics of fertility segregation at heading stage. In a total of 963 F2 populations, there were 715 fertile individuals and 248 sterile individuals, meeting the genetic segregation ratio of 3 (fertile): 1 (sterile) (χ 2 =0.29<3.84), indicating that low-temperature-sensitive genic sterility traits are controlled by a single recessive gene.

Embodiment 2

[0024] Example 2 Genetic Mapping and Marker Development of Gene rtms10

[0025] DNA pools of fertile individual plants and sterile individual plants were respectively constructed, and a low-temperature-sensitive genic male sterile gene was located on chromosome 10 by Bulked Segregant Analysis, named rtms10, and the expression of the gene was developed. The associated molecular marker ID1305, and the primer sequences are shown in the table below.

[0026]

Embodiment 3

[0027] Molecular cultivation of embodiment 3 inverse thermosensitive genic male sterile line

[0028] Fertile parents Samsara 422, LR009 and Peiai 64 with excellent comprehensive agronomic traits were selected as recurrent parents, crossed with Yannong S, backcrossed for 3 to 4 generations and selfed. Marker ID1305 was used in each segregation generation (PCR reaction system: 50ng DNA; 5ul TAKARA rTaq buffer; 2ul each of upstream and downstream primers at a concentration of 10mM; 5ul of dNTPs at a concentration of 2.5mM; 30sec, 32 cycles; 72°C for 5min) Individual plants and lines carrying the rtms10 gene and excellent agronomic traits were selected and bred, and a practical reverse temperature-sensitive genic male sterile line with excellent agronomic traits was obtained. Among them, the molecular marker ID1305 on YNS and L422 F 2 See the expansion results of low-temperature sterile plants in the isolated population figure 2 , M: DNA marker; L: Samsara 422; Y: YNS; 1-38: F...

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Abstract

The invention belongs to the technical fields of crop genetics and breeding, and particularly relates to a molecular marker of a low-temperature-sensitive genic male sterile gene and application thereof. According to the molecular marker of the low-temperature-sensitive genic male sterile gene, a reverse-temperature-sensitive genic male sterile resource, namely a Yannong-S low-temperature-sensitive genic male sterile gene rtms10, is located on chromosome 10 of rice through genetic analysis; and a related molecular marker ID1305 is developed. Then, a fertile parent is selected as a recurrent group, and is cross-bred, back-crossed and self-bred with the Yannong-S low-temperature-sensitive genic male sterile gene rtms10; and a reverse-temperature-sensitive genic male sterile line is selectedand bred by utilizing a molecular marker selection means. Amplified fragments of the developed molecular marker ID1305 in the low-temperature-sensitive genic male sterile gene rtms10 is 159 bp; and the amplified fragments of the developed molecular marker ID1305 in normal fertile materials is 180 bp. On basis of differences of amplification products in the reverse-temperature-sensitive genic malesterile line and the normal materials, the molecular marker ID1305 can be used for identifying purity of two-line hybrid rice combined with the reverse-temperature-sensitive genic male sterile line, as well as for molecule selecting and breeding of the reverse-temperature-sensitive genic male sterile line.

Description

technical field [0001] The invention belongs to the technical field of crop genetic breeding, and in particular relates to a molecular marker of a low-temperature-sensitive sterility gene and an application thereof. Background technique [0002] The discovery of rice light- and temperature-sensitive sterile resources (two-line sterile resources) and its successful application in hybrid rice have played an important role in ensuring food security in my country. Compared with the three-line CMS, the two-line CMS has the advantages of self-propagation and wide recovery spectrum. Therefore, the two-line hybrid rice has developed rapidly in my country in recent years, and the planting area and promotion range have expanded rapidly. However, the promotion of two-line hybrid rice also faces many difficulties such as high risk of seed production, narrow breeding area of ​​sterile lines, and single type of practical sterile resources. [0003] The discovery of light- and temperature...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 倪金龙倪大虎宋丰顺杨剑波王德正钱征林翠香
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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