Dynamic cell culture method and device for simulating dynamic environment in vivo

Abstract

The invention relates to a dynamic cell culture method and device for simulating the dynamic environment in vivo. The dynamic cell culture method comprises the steps that cells are attached to a flexible substrate in vitro for culture, a cyclic dynamic load with a certain frequency and a certain direction is applied to the flexible substrate in the culture process, in all cyclic periods, the loadis increased gradually along with the time first until reaches a largest value, and then is gradually decreased along with the time, and the deformation rate of the flexible substrate varies with theload synchronously, is increased linearly first, and then is decreased linearly; the culture device comprises a cell culture room, a clamping device for clamping the flexible substrate, a pressing head for applying the load to the flexible substrate, and a center aligning type crank sliding block mechanism for driving the pressing head to make reciprocating motion in the direction perpendicular tothe flexible substrate, and the clamping device and the pressing head are located in the cell culture room. According to the dynamic cell culture method, the interactive relationship of a flexible implant-cell in vivo can be accurately simulated; and the culture device is easy and convenient to assemble, using is convenient, and the culture device extremely has generalizing value.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to a dynamic cell culture method and a culture device for simulating a dynamic environment in a body. Background technique [0002] In vitro cell culture is of great significance for judging the growth and development of cells after material implantation in vivo, and the integration performance of materials and tissues. In vitro cell culture refers to the growth, development and migration of cells in an in vitro environment by simulating the in vivo environment. Compared with animal experiments and in vivo experiments, in vitro cell culture has the advantages of low cost, simple operation, easy observation and no ethical issues. However, there are still many shortcomings in in vitro cell culture. In vitro cell culture is mainly static culture, including culture bottle method, culture dish method, culture plate method, etc., which cannot accurately simulate the in vivo environmen...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that in vitro cell culture cannot accurately simulate the internal environment, especially the dynamic environment under different stress conditions in the prior art, and provide a dynamic cell culture method and a culture device for simulating the dynamic environment in the body, especially provide a An in vitro dynamic cell culture method and culture device that simulates the axial and radial mechanical stimulation of flexible substrates in vivo, the method can simulate the in vivo environment during in vitro culture, so that cells can be cultured under conditions that are more suitable for the in vivo environment , the data obtained are closer to the actual in vivo situation. Compared with in vivo experiments, the experimental cost of this method is greatly reduced, and the culture device used in this method is easy to operate, and different parameters can be set according to needs.

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