Fixed-bed bioreactor system for large-scale production of mesenchymal stem cells

Abstract

The invention discloses a fixed-bed bioreactor system for large-scale production of mesenchymal stem cells. The system comprises a bioreactor (1), an outer circulation mechanism (2) and a collecting mechanism (3), wherein the bioreactor (1) is used for amplifying the mesenchymal stem cells; the outer circulation mechanism (2) is used for receiving a culture medium discharged from the bottom of thebioreactor (1) and conveying the culture medium to a culture medium mixing tank (11) at the upper part of the bioreactor (1) to realize circulation of the culture medium and is internally provided with a flow meter (22) to monitor a flow velocity of the culture medium and regulate substance exchange of the culture medium according to the flow velocity; and the collecting mechanism (3) can collectthe mesenchymal stem cells cultured by the bioreactor (1) and waste liquid produced during culture of the mesenchymal stem cells. For the fixed-bed bioreactor system provided by the invention, through external circulation of the culture medium, the flow velocity of the culture medium passing through a fixed bed can be accurately controlled, so that large-scale culture of the mesenchymal stem cells is realized.

Description

technical field [0001] The invention belongs to the technical field of mesenchymal stem cell culture, in particular to the technical field of large-scale production of mesenchymal stem cells, in particular to a fixed-bed bioreactor system for large-scale production of mesenchymal stem cells. Background technique [0002] The traditional fixed bed reactor generally adopts the circulation in the tank. During the circulation in the tank, the rotation of the stirring paddle drives the circulation of the medium in the tank, but the flow rate of the medium passing through the carrier during the circulation cannot be measured, so it is difficult to measure The nutrient transfer and shear force of stem cells are effectively controlled, making it difficult to improve the quality of stem cell culture and to carry out large-scale production. At present, there are two stirring forms for fixed bed reactors, one is upward stirring and the other is downward stirring, but either way, there ...

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Problems solved by technology

[0002] The traditional fixed bed reactor generally adopts the circulation in the tank. During the circulation in the tank, the rotation of the stirring paddle drives the circulation of the medium in the tank, but the flow rate of the medium passing through the carrier during the circulation cannot be measured, so it is difficult to measure The nutrient transfer and shear force of stem cells are effectively controlled, making it difficult to improve the quality of stem cell culture and large-scale production

At present, there are two stirring forms for fixed bed reactors, one is upward stirring and the other is downward stirring, but either way, there is only one stirring paddle, and the bottom of the tank adopts a spherical head. The volume of the type head is large, resulting in a large amount of digestive juice residue, affecting the next growth of adherent cells, resulting in waste and low efficiency

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