Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of scorpion toxin active polypeptide hstx1 with improving cerebral ischemia and hypoxia and its preparation method and application

A technology of active polypeptide and cerebral ischemia, which is applied in the field of biomedicine to achieve the effect of strongly improving the activity of motor dysfunction, improving strong activity, and high efficiency and strong activity

Active Publication Date: 2022-07-01
KUNMING MEDICAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, active peptides that improve cerebral ischemia and hypoxia are rarely reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of scorpion toxin active polypeptide hstx1 with improving cerebral ischemia and hypoxia and its preparation method and application
  • A kind of scorpion toxin active polypeptide hstx1 with improving cerebral ischemia and hypoxia and its preparation method and application
  • A kind of scorpion toxin active polypeptide hstx1 with improving cerebral ischemia and hypoxia and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The preparation method of the scorpion toxin active polypeptide HsTx1 with improving cerebral ischemia and hypoxia according to the present invention comprises the following steps:

[0029] A. Take the stinger secretion that is hooked on the tail end of the tropical rain forest scorpion by electrical stimulation, dissolve it in PBS, obtain material a after vacuum freeze drying, and store it at -80°C for later use;

[0030] B, material a is dissolved in water to obtain material b, and material b is eluted with Tris-HCl buffer, and the eluate is collected to obtain material c;

[0031] C, material c is carried out for the first time high-performance liquid chromatography reversed-phase chromatography to obtain material d;

[0032] D. Perform the second high-performance liquid chromatography reversed-phase chromatography on the material d to obtain the target substance HsTx1, which has the scorpion toxin active polypeptide HsTx1 that improves cerebral ischemia and hypoxia....

Embodiment 1

[0043] Isolation, purification and identification of scorpion toxin active polypeptide HsTx1 with improved cerebral ischemia and hypoxia

[0044] 1. Separation and purification

[0045] The living tropical rain forest scorpion was collected from Xishuangbanna, Yunnan, and the stinger secretions (dissolved in PBS) were collected by electrical stimulation, and the stinger secretions (dissolved in PBS) were obtained.

[0046] The first step: Sephadex G75 molecular sieve:

[0047] The lyophilized powder of skin secretions was obtained according to the above method, dissolved in deionized water, and 500 μl (protein content of 100 mg) was pre-equilibrated with 20 mM Tris-HCl buffer (pH=7.8, containing 0.1 M NaCl) for 24 h A Sephadex G75 (GE Healthcare, ultra-fine) column (length 40 cm, id width 1.5 cm) was eluted with the same buffer at a flow rate of 1.5 ml / 10 min, collected every 10 min, and measured at 280 nm absorbance.

[0048] The second step: the first high-performance liq...

Embodiment 2

[0056] Anti-cerebral ischemia-hypoxia activity of scorpion toxin active polypeptide HsTx1 that improves cerebral ischemia and hypoxia

[0057] In the present invention, the cerebral ischemia and hypoxia animal model (MCAO) experiment is used to detect the anti-cerebral ischemia and hypoxia activity of HsTx1. , and can effectively improve the dysfunction after cerebral ischemia and hypoxia.

[0058] SPF male SD rats weighing 250-280 g were selected for the experiment, and the rats were randomly divided into three groups: sham operation group, MCAO control group, MCAO+1 nmol / kg group, MCAO+2 nmol / kg group, MCAO+ 5 nmol / kg group, 7 rats in each group, and the rats were trained on balance beam before operation. First, rats were anesthetized by intraperitoneal injection of 3% sodium pentobarbital (30 mg / kg). % alcohol was used for local disinfection, and then the skin was cut at the outer 1 / 3 of the line connecting the lateral canthus of the right eye and the external auditory ca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia, and a preparation method and application thereof. The amino acid sequence of the scorpion toxin active polypeptide HsTx1 with improving cerebral ischemia and hypoxia is AGKKERAGSRRTKIVMLKCIREHGH. The tropical rainforest scorpion toxin polypeptide HsTx1 purified by the present invention shows strong anti-cerebral ischemia and hypoxia (MCAO) activities and improves motor dysfunction in the cerebral ischemia and hypoxia model (MCAO) experiment, indicating that the "scorpion toxin of the present invention can improve cerebral ischemia and hypoxia." Hypoxic active polypeptide HsTx1" has great medical application prospects. The scorpion toxin active polypeptide HsTx1 with improving cerebral ischemia and hypoxia described in the present invention has the beneficial characteristics of strong activity of effectively improving cerebral ischemia hypoxia and motor dysfunction.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia, and a preparation method and application thereof. Background technique [0002] As we all know, cerebral ischemia is one of the most common acute cerebrovascular diseases, and has been recognized as the third leading cause of mortality and disability in the world, causing huge economic and psychological burdens on individuals and socioeconomics. Acute cerebrovascular disease is a group of diseases that cause brain tissue damage, including ischemic and hemorrhagic stroke, due to the sudden rupture of blood vessels in the brain or the inability of blood to flow to the brain due to blockage of the blood vessels. Among them, the incidence of ischemic stroke is high, accounting for 60% to 70% of the total number of strokes, and its symptoms are unilateral head and face, sudden...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/14C07K1/20A61K38/17A61P9/10A23L33/18
CPCC07K14/43522A61P9/10A23L33/18A61K38/00A23V2002/00A23V2200/30A23V2250/55
Inventor 杨新旺孙俊杨美凤王滢宋咏丽尹赛格
Owner KUNMING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com