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A Strain of Anaerobic Bacillus spp. and Its Application

An aerobic bacillus and aerobic bacillus technology, applied in the field of microorganisms, can solve the problems of low extraction rate, secondary pollution caused by the environment, and high processing cost, and achieve the effects of high recovery rate, small secondary pollution and low cost

Active Publication Date: 2021-04-27
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is in view of the traditional method of processing heavy metal-containing wastewater that is likely to cause secondary pollution to the environment, and has high processing costs and low extraction rates, and the recovery of heavy metals has huge economic value. The present invention provides a Kind of anaerobic Bacillus thermoflavinum and its application

Method used

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  • A Strain of Anaerobic Bacillus spp. and Its Application
  • A Strain of Anaerobic Bacillus spp. and Its Application
  • A Strain of Anaerobic Bacillus spp. and Its Application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: Isolation, cultivation and growth of anaerobic spores

[0032] Prepare 30 mL of sterile water (sterilize the deionized water at 121°C for 21 minutes in a high-temperature sterilizer), and mix the collected hot spring water and soil samples (the sampling location is Gui’an Village, Lianjiang County, Fuzhou City, Fujian Province, and the sampling time is 2018 March 24, 2010) poured into sterile water, then shaken to mix and take the supernatant in LB liquid medium (tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L), placed in 60 Cultivate at °C, after the liquid medium is turbid, take 100uL suspension (diluted with different concentration gradients) and apply it on LB solid medium (tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, agar powder 20g / L), cultured at 60°C, and when a single colony grows in the solid medium, pick a single colony and separate it by streaking to obtain a pure culture of anaerobic spore spores. The growth curve of t...

Embodiment 2

[0036] Example 2: Identification and Morphological Observation of Phytophthora anaerobic Bacillus

[0037] Experimental Bacteria Genome DNA Extraction Kit Extraction Example 1 Isolation and Obtaining the Genome of Anaerobic Bacillus Pyroflavinus, Using Universal Primers 27F: 5'-AGAGMTGATCATGGCTCAG-3' and 1492R: 5'-GGGYTACCTTGTTACGACTT-3' of Bacterial 16S rRNA Gene , the 16SrRNA gene of the strain was amplified by PCR using the genomic DNA of Anaerobic Bacillus japonica as a template. The PCR reaction conditions were: 95°C for 5min; 95°C for 30s, 55°C for 30s, 72°C for 90s, cycle 30 times; 72°C for 10min. The PCR products were gel-cut and recovered after 1% agarose electrophoresis for sequence determination. The sequence of the 16S rRNA gene of the anaerobic bacterium Pyrrhus flavinus obtained by sequencing is shown in SEQ ID NO.1.

[0038] Construction and analysis of the phylogenetic tree: the 16S rRNA gene sequence of the anaerobic Bacillus euthermus strain was compared wi...

Embodiment 3

[0040] Embodiment 3: Optimum temperature and pH of the growth of anaerobic bacterium Pyritus japonica

[0041] Inoculate the anaerobic Bacillus japonica into LB medium, culture at 60°C until OD 600 =0.1 to obtain the seed solution, the seed solution was inoculated in LB medium with different pH (pH=4, 5, 6, 7, 8, 9, 10) according to the inoculation amount of 3wt%, and then placed in different temperature conditions for static culture (T=55, 60, 65, 70, 75°C), after 12 hours of cultivation, observe the growth of the anaerobic spores of the pyrophorus and the OD values ​​of the anaerobic spores of the pyrogens under different temperature and pH conditions, respectively See Figure 5 , 6 . From Figure 5 It can be seen that the surviving pH of anaerobic Bacillus xanthus is 5-9, and it grows best under neutral and slightly acidic conditions (6-7). From Figure 6 It can be known that the tolerant maximum temperature of the anaerobic bacterium Pyritus japonica is 65°C, and it ...

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Abstract

The present invention provides a strain of anaerobic bacillus eupyroflavitas and application thereof, and the strain is anaerobic bacterium eupyroflavitas ( Anoxybacillus flavithermus ) Fzu200, which was registered and preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on December 24, 2018. The preservation number is: CGMCC No.17024. Recovery of heavy metal nickel in high temperature factory wastewater and high temperature mine wastewater. Compared with the traditional heavy metal treatment method, the method has less secondary pollution, low cost and high recovery rate.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an anaerobic bacillus euthermus and its application. Background technique [0002] With the development of social economy, the demand for metals in human society has also risen sharply, and the resulting heavy metal wastes (such as mercury (mercury), nickel, cadmium, lead, and chromium, etc.) are also increasing. The waste slag and waste water produced in the process of heavy metal mining are also a big problem that needs to be solved urgently. In the process of mining, some heavy metal-containing minerals are easy to enter the water body and soil through precipitation and other means, and then release heavy metal ions into the environment under biological activities such as microorganisms. However, some small factories (metal smelters and manufacturing plants, etc.) are greedy for economic interests, and the waste water is not treated, and is directly discharged in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20C02F103/10
CPCC02F3/34C02F2101/20C02F2103/10C12N1/20C12N1/205C12R2001/01
Inventor 程扬健钟超柯志滨
Owner FUZHOU UNIV
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