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A method for improving the quality of double-strand arrowroot tissue culture seedlings

A technology of tissue culture seedlings and arrowroot, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems affecting the proliferation, differentiation and rooting of bottle seedlings, irregular plant growth, and low survival rate of seedlings, etc., to achieve The effect of improving reproductive efficiency, reducing yellow leaf rate and mutation rate, and increasing induction rate of tissue culture

Active Publication Date: 2022-06-07
广州市名卉景观科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional propagation methods of double-lined arrowroot generally include division propagation and cutting propagation, but these two propagation methods have low reproduction coefficient, slow production speed, uneven plant growth, and are difficult to mass-produce
[0003] The current double-line arrowroot tissue culture technology is prone to yellow leaves, which not only affects the proliferation, differentiation and rooting of bottle seedlings, but also causes problems such as scorched leaves after hardening and transplanting, low survival rate of seedlings, and long recovery time.

Method used

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  • A method for improving the quality of double-strand arrowroot tissue culture seedlings

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Experimental program
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Effect test

Embodiment 1

[0024] A method for improving the quality of arrowroot tissue culture seedlings, comprising: S11. taking the side buds of the mother plant of arrowroot growing underground, cleaning and removing the substrate, and removing the roots; S12. cleaning the side buds and drying them until the surface is dry, After wiping with alcohol, add it to alcohol for sterilization for 0.5~1min, then add the large buds in the lateral buds to sodium hypochlorite solution for sterilization for 40~50min, and clean them to obtain explants; S13. Induce adventitious buds on the explants Cultivate until adventitious buds are obtained; S14. Remove the top and base of the adventitious buds, and separate the new shoots grown on the adventitious buds to obtain new shoots and original shoots, and subculture the new shoots and original shoots to obtain multiple robust For adventitious buds, the adventitious buds are propagated; S15. Divide the robust adventitious buds into single buds, and after rooting and ...

Embodiment 2

[0027] The difference between Example 2 and Example 1 is that, in the step S14, a proliferation medium is selected when carrying out the adventitious bud proliferation culture, and the proliferation medium further comprises: NH 4 NO 3 825~1650mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O 740~925mg / L, KH 2 PO 4 170~340mg / L, CaCl 2 ·2H 2 O 110~220mg / L, KI 0.42~0.83mg / L, H 3 BO 3 3.1~6.2mg / L, MnSO 4 ·4H 2 O 16.9~33.8mg / L, ZnSO 4 ·7H 2 O 4.3~8.6mg / L, Na 2 MoO 4 ·2H 2 O 0.13~0.25mg / L, CuSO 4 ·5H 2 O 0.013~0.025mg / L, CoCl 2 ·6H 2 O 0.013~0.025mg / L, FeSO 4 ·7H 2 O13.9~55.6mg / L, Na 2 ·EDTA 18.7~74.6mg / L, thiamine hydrochloride 0.1mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2mg / L, inositol 100mg / L, sugar 30g / L , Carrageenan 6.2g / L, 6-benzylaminopurine 0.5~1.0mg / L, naphthalene acetic acid 0.05~0.1mg / L.

Embodiment 3

[0029] The difference between Example 3 and Example 1 is that in the step S14, a proliferation medium is selected when carrying out the adventitious bud proliferation culture, and the proliferation medium further comprises: NH 4 NO 3 1650mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O 185~740mg / L, KH 2 PO 4 170mg / L, CaCl 2 ·2H 2 O 220~880mg / L, KI 0.42~0.83mg / L, H 3 BO 3 3.1~6.2mg / L, MnSO 4 ·4H 2 O 8.5~15mg / L, ZnSO 4 ·7H 2 O 4.3~8.6mg / L, Na 2 MoO 4 ·2H 2 O 0.13~0.25mg / L, CuSO 4 ·5H 2 O 0.013~0.025mg / L, CoCl 2 ·6H 2 O 0.013~0.025mg / L, FeSO 4 ·7H 2 O13.9~55.6mg / L, Na 2 ·EDTA 18.7~74.6mg / L, thiamine hydrochloride 0.1mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2mg / L, inositol 100mg / L, sugar 30g / L , Carrageenan 6.2g / L, 6-benzylaminopurine 1.5~2.0mg / L, naphthalene acetic acid 0.15~0.3mg / L.

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Abstract

The invention relates to the field of double-strand arrowroot planting, and provides a method for improving the quality of double-strand arrowroot tissue-cultured seedlings, which is used to solve the problem of yellowing leaves of the double-strand arrowroot tissue-cultured seedlings. A method for improving the quality of arrowroot tissue culture seedlings provided by the invention comprises: S11. taking the lateral buds of the parent plant of arrowroot growing underground, cleaning and removing the matrix, and removing the roots; S12. cleaning the lateral buds and drying them Dry the surface, clean it after disinfection and sterilization, and obtain explants; S13. Induce and cultivate adventitious buds on the explants to obtain adventitious buds; S14. Proliferate and cultivate the induced adventitious buds to a certain number; S15. Divide the robust adventitious buds into single buds, and after rooting and culturing the single buds for a certain period of time, the tissue cultured seedlings that can be transplanted are obtained. During the cultivation process, the survival rate of the seedlings is high, the reproduction efficiency is greatly improved, the yellow leaf rate of the plants can be reduced, and the growth cycle of the tissue culture seedlings after transplanting can be shortened.

Description

technical field [0001] The invention relates to the field of double-line arrowroot planting, in particular to a method for improving the quality of double-line arrowroot tissue culture seedlings. Background technique [0002] Double-line arrowroot ( Calathea sanderiana ), arrowroot family, arrowroot is a perennial evergreen herbaceous ornamental plant, originating in Brazil and other South and Central America places. Likes warm, humid and semi-shaded environments. The leaves are basal or cauline, the leaves are oblong, with white bands and dark green bands alternately arranged on both sides of the main vein in a pinnate arrangement, and the leaves have a bright color contrast. Because of the beautiful plant shape, high ornamental value, and strong shade tolerance, Widely used, it is a famous foliage plant, and the market demand has maintained a good momentum. The traditional propagation methods of arrowroot are generally divided into two methods: division propagation and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 马文卿黄国涛曾日卿江碧玉阮燕珠卢钦旺
Owner 广州市名卉景观科技发展有限公司
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