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Method for improving quality of lacy tree philodendron tissue culture seedlings

A technology of tissue culture seedlings and arrowroot, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems affecting the proliferation, differentiation and rooting of bottle seedlings, irregular plant growth, and low survival rate of seedlings, and achieve the goal of breeding Improve efficiency, reduce yellow leaf rate and variation rate, and reduce the effect of yellow leaf rate

Active Publication Date: 2019-11-22
广州市名卉景观科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional propagation methods of double-lined arrowroot generally include division propagation and cutting propagation, but these two propagation methods have low reproduction coefficient, slow production speed, uneven plant growth, and are difficult to mass-produce
[0003] The current double-line arrowroot tissue culture technology is prone to yellow leaves, which not only affects the proliferation, differentiation and rooting of bottle seedlings, but also causes problems such as scorched leaves after hardening and transplanting, low survival rate of seedlings, and long recovery time.

Method used

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  • Method for improving quality of lacy tree philodendron tissue culture seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for improving the quality of double-strand arrowroot tissue culture seedlings, comprising:

[0030] S11. Get the lateral buds of the double-line arrowroot mother plant growing underground, clean and remove the matrix, and remove the roots;

[0031] S12. Clean the lateral buds, dry them until the surface is dry, wipe them with alcohol and add them to alcohol for sterilization for 0.5-1min, then add the large buds in the lateral buds to the sodium hypochlorite solution to continue the sterilization for 40-50min, clean them, and get Explant;

[0032] S13. Carry out adventitious bud induction culture on the explant until obtaining adventitious bud;

[0033] S14. remove the top and base of the adventitious buds, and separate the new shoots grown on the adventitious buds to obtain the new shoots and the original buds, and carry out the subgeneration of the new shoots and the original buds until obtaining a plurality of robust adventitious buds. multiply;

[0034]S...

Embodiment 2

[0037] The difference between embodiment 2 and embodiment 1 is that in the S14 step, the proliferation medium is selected for adventitious bud proliferation culture, and the proliferation medium also includes: NH 4 NO 3 825~1650mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O 740~925mg / L, KH 2 PO 4 170~340mg / L, CaCl 2 2H 2 O 110~220mg / L, KI 0.42~0.83mg / L, H 3 BO 3 3.1~6.2mg / L, MnSO 4 · 4 h 2 O 16.9~33.8mg / L, ZnSO 4 ·7H 2 O 4.3~8.6mg / L, Na 2 MoO 2 2H 2 O 0.13~0.25mg / L, CuSO 4 ·5H 2 O 0.013~0.025mg / L, CoCl 2 ·6H 2 O 0.013~0.025mg / L, FeSO 4 ·7H 2 O13.9~55.6mg / L, Na 2 EDTA 18.7~74.6mg / L, thiamine hydrochloride 0.1mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2mg / L, inositol 100mg / L, sugar 30g / L , Carrageenan 6.2g / L, 6-benzylaminopurine 0.5~1.0mg / L, naphthaleneacetic acid 0.05~0.1mg / L.

Embodiment 3

[0039] The difference between embodiment 3 and embodiment 1 is that in the S14 step, the proliferation medium is selected for adventitious bud proliferation culture, and the proliferation medium also includes: NH 4 NO 3 1650mg / L, KNO 3 1900mg / L, MgSO 4 ·7H 2 O 185~740mg / L, KH 2 PO 4 170mg / L, CaCl 2 2H 2 O 220~880mg / L, KI 0.42~0.83mg / L, H 3 BO 3 3.1~6.2mg / L, MnSO 4 · 4 h 2 O 8.5~15mg / L, ZnSO 4 ·7H 2 O 4.3~8.6mg / L, Na 2 MoO 2 2H 2 O 0.13~0.25mg / L, CuSO 4 ·5H 2 O 0.013~0.025mg / L, CoCl 2 ·6H 2 O 0.013~0.025mg / L, FeSO 4·7H 2 O 13.9~55.6mg / L, Na 2 EDTA 18.7~74.6mg / L, thiamine hydrochloride 0.1mg / L, niacin 0.5mg / L, pyridoxine hydrochloride 0.5mg / L, glycine 2mg / L, inositol 100mg / L, sugar 30g / L , carrageenan 6.2g / L, 6-benzylaminopurine 1.5~2.0mg / L, naphthaleneacetic acid 0.15~0.3mg / L.

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Abstract

The invention relates to the field of lacy tree philodendron planting and provides a method for improving the quality of lacy tree philodendron tissue culture seedlings. The method for improving the quality of lacy tree philodendron tissue culture seedlings is used for solving the problem of possible leaf yellowing of lacy tree philodendron tissue culture seedlings. The method for improving the quality of lacy tree philodendron tissue culture seedlings comprises the following steps: S11, taking lateral buds growing underground of lacy tree philodendron stock plants, executing cleaning to remove a matrix, and removing roots; S12, cleaning the lateral buds, executing air drying until the surfaces are dry, executing cleaning after sterilization to obtain explants; S13, conducting adventitiousbud induction culture on the explants until adventitious buds are obtained; S14, conducting propagating culture on the induced adventitious buds to achieve a certain amount of expanding propagation;S15, dividing healthy and strong adventitious buds into single buds, and carrying out rooting culture on the single buds for a certain time to obtain tissue culture seedlings which can be transplanted. The survival rate of the seedlings in the culture process is high, the propagation efficiency is greatly improved, the occurrence of the leaf yellowing rate of the plants can be reduced, and the growth period of the tissue culture seedlings after transplantation is shortened.

Description

technical field [0001] The invention relates to the field of double-strand arrowroot planting, in particular to a method for improving the quality of double-strand arrowroot tissue culture seedlings. Background technique [0002] Double lined arrowroot ( Calathea sanderiana ), Arrowroot, Arrowroot is a perennial evergreen herbaceous foliage plant, originating in South and Central America such as Brazil. Likes warm, humid and semi-shaded environments. The leaves are basal or cauline, and the leaves are oblong. On both sides of the main vein, there are white bands and dark green bands alternately arranged in a pinnate shape. The color of the leaves is contrasting. Widely used, it is a famous foliage plant, and the market demand has maintained a good momentum. The traditional propagation methods of double-strand arrowroot generally include division propagation and cutting propagation, but these two propagation methods have low reproduction coefficient, slow production speed,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 马文卿黄国涛曾日卿江碧玉阮燕珠卢钦旺
Owner 广州市名卉景观科技发展有限公司
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