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A kind of transportation method of sugarcane tissue culture rooted shoot

A transportation method and technology for sugarcane, applied in sugarcane cultivation, horticultural methods, botanical equipment and methods, etc., can solve the problems of high cost and low survival rate of false planting, reduce root rot, prolong storage time, and reduce transportation cost. Effect

Active Publication Date: 2021-05-11
广东省科学院南繁种业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for reducing the transportation cost of sugarcane tissue culture rooted seedlings and improving the transportation cost of the existing sugarcane tissue culture rooted seedlings. Sugarcane Tissue Culture Rooted Seedling Transportation Method for Survival Rate of False Plants

Method used

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  • A kind of transportation method of sugarcane tissue culture rooted shoot
  • A kind of transportation method of sugarcane tissue culture rooted shoot
  • A kind of transportation method of sugarcane tissue culture rooted shoot

Examples

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Effect test

Embodiment 1

[0029] 45,000 healthy black-skinned fruit cane seedlings were bred from the Guangzhou Sugarcane Sugar Industry Research Institute. In March 2015, the seedlings were transported to Shaoguan Wengyuan sugarcane area for artificial planting. Follow the steps below:

[0030] 1) Acquisition of sugarcane tissue culture rooted seedlings: the disease-free black-skinned fruit cane propagation bottle seedlings obtained by cutting out 1-2mm growth points of sugarcane buds through hot water treatment and 40°C high-temperature germination, and culturing them are inserted into root-promoting culture Base: 1 / 2MS+sucrose 50000mg / L+IBA 2.0mg / L+NAA 1.0mg / L+paclobutrazol 1.0mg / L, light intensity 2000Lux, light time 16 hours a day, temperature 27℃. After 25 days of root-promoting culture, it can be seen that the sugarcane tissue culture seedlings grow whiter and healthy roots.

[0031] 2) Pretreatment of rooted seedlings before transportation: Take out the rooted bottle seedlings of black-skin fru...

Embodiment 2

[0034] 38,000 healthy yellow-skinned cane seedlings were bred from the Guangzhou Sugarcane Sugar Industry Research Institute, and they were transported to Shaoguan Wengyuan Sugarcane Area for artificial planting in March 2016. The steps are as follows:

[0035] 1) Acquisition of sugarcane tissue culture rooted seedlings: the disease-free yellow-skinned fruit cane propagation bottle seedlings obtained by cutting out 1-2 mm growth points of sugarcane buds through hot water treatment and 40°C high-temperature acceleration, and culturing them, are inserted into root-promoting culture Base: 1 / 2MS+sucrose 50000mg / L+IBA 2.5mg / L+NAA 1.5mg / L+paclobutrazol 1.2mg / L, light intensity 2000Lux, light time 16 hours a day, temperature 27°C. After 20 days of root-promoting culture, it can be seen that the sugarcane tissue culture seedlings grow whiter and healthy roots.

[0036] 2) Pretreatment of rooted seedlings before transportation: Take out the yellow-skin fruit cane rooted bottle seedling...

Embodiment 3

[0039] 40,000 sugarcane Yuetang No. 60 tissue culture rooting seedlings were bred from the Guangzhou Sugarcane Sugar Industry Research Institute in Guangzhou. In May 2017, they were transported to Maoyuan Sugar Factory in Wengyuan, Shaoguan City for artificial planting. The following steps were taken:

[0040] 1) Acquisition of sugarcane tissue culture rooted seedlings: the disease-free Yuetang No. 60 propagation bottle seedlings obtained by hot water treatment and 40°C high-temperature germination, cutting out 1-2 mm growth points of sugarcane buds for cultivation, and inserting root-promoting culture Base: 1 / 2MS+sucrose 50000mg / L+IBA 2.5mg / L+NAA 1.5mg / L+paclobutrazol 1.5mg / L, light intensity 2000Lux, light time 16 hours a day, temperature 28°C. After 25 days of root-promoting culture, it can be seen that the sugarcane tissue culture seedlings grow whiter and healthy roots.

[0041] 2) Pretreatment of rooted seedlings before transportation: take out the domesticated and harde...

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Abstract

The invention discloses a method for transporting rooted sugarcane tissue cultured seedlings, which comprises the following steps: obtaining rooted sugarcane tissue cultured rooted seedlings, pretreatment of rooted seedlings before transportation, and outdoor artificial planting; during transportation, acrylamide treated with sodium benzoate is used. Acrylate crosslinked copolymer is used as a protective matrix, and the transportation method of sugarcane tissue culture rooted shoots can greatly reduce the transportation cost of sugarcane tissue culture rooted shoots and improve the survival rate of false plants. The combined use of sodium benzoate at a high concentration can provide a good protective effect on sugarcane tissue culture seedlings, and greatly improve the survival rate of sugarcane tissue culture seedlings after long-distance transportation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for transporting sugarcane tissue culture rooted seedlings. Background technique [0002] Sugarcane tissue culture is a high-efficiency propagation technique, and using sugarcane shoot apex meristem culture is an effective way to obtain disease-free seedlings. However, the production base of sugarcane disease-free seedlings is often not in the same district or city as the farm or sugarcane field that needs to be planted and planted. Farm or sugarcane field. The seedlings are packed in culture bottles for transportation, although the damage to the seedlings is small, the volume and weight of the bottle seedlings that need to be transported are greatly increased, and the transportation cost is increased. If the culture bottle is removed and only the tissue-cultured rooted seedlings are transported, although the transportation cost can be redu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/55
CPCA01H4/001A01H4/008A01G22/55
Inventor 何慧怡齐永文樊丽娜劳方业陈勇生
Owner 广东省科学院南繁种业研究所
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