Method for transporting sugarcane tissue culture rooting seedlings
A transportation method and sugarcane technology, applied in sugarcane cultivation, horticultural methods, botanical equipment and methods, etc., can solve the problems of low survival rate and high cost of fake plants
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Embodiment 1
[0029] 45,000 healthy black-skinned fruit cane seedlings were bred from the Guangzhou Sugarcane Sugar Industry Research Institute. In March 2015, the seedlings were transported to Shaoguan Wengyuan sugarcane area for artificial planting. Follow the steps below:
[0030] 1) Acquisition of sugarcane tissue culture rooted seedlings: the disease-free black-skinned fruit cane propagation bottle seedlings obtained by cutting out 1-2mm growth points of sugarcane buds through hot water treatment and 40°C high-temperature germination, and culturing them are inserted into root-promoting culture Base: 1 / 2MS+sucrose 50000mg / L+IBA 2.0mg / L+NAA 1.0mg / L+paclobutrazol 1.0mg / L, light intensity 2000Lux, light time 16 hours a day, temperature 27℃. After 25 days of root-promoting culture, it can be seen that the sugarcane tissue culture seedlings grow whiter and healthy roots.
[0031] 2) Pretreatment of rooted seedlings before transportation: Take out the rooted bottle seedlings of black-skin fru...
Embodiment 2
[0034] 38,000 healthy yellow-skinned cane seedlings were bred from the Guangzhou Sugarcane Sugar Industry Research Institute, and they were transported to Shaoguan Wengyuan Sugarcane Area for artificial planting in March 2016. The steps are as follows:
[0035] 1) Acquisition of sugarcane tissue culture rooted seedlings: the disease-free yellow-skinned fruit cane propagation bottle seedlings obtained by cutting out 1-2 mm growth points of sugarcane buds through hot water treatment and 40°C high-temperature acceleration, and culturing them, are inserted into root-promoting culture Base: 1 / 2MS+sucrose 50000mg / L+IBA 2.5mg / L+NAA 1.5mg / L+paclobutrazol 1.2mg / L, light intensity 2000Lux, light time 16 hours a day, temperature 27°C. After 20 days of root-promoting culture, it can be seen that the sugarcane tissue culture seedlings grow whiter and healthy roots.
[0036] 2) Pretreatment of rooted seedlings before transportation: Take out the yellow-skin fruit cane rooted bottle seedling...
Embodiment 3
[0039] 40,000 sugarcane Yuetang No. 60 tissue culture rooting seedlings were bred from the Guangzhou Sugarcane Sugar Industry Research Institute in Guangzhou. In May 2017, they were transported to Maoyuan Sugar Factory in Wengyuan, Shaoguan City for artificial planting. The following steps were taken:
[0040] 1) Acquisition of sugarcane tissue culture rooted seedlings: the disease-free Yuetang No. 60 propagation bottle seedlings obtained by hot water treatment and 40°C high-temperature germination, cutting out 1-2 mm growth points of sugarcane buds for cultivation, and inserting root-promoting culture Base: 1 / 2MS+sucrose 50000mg / L+IBA 2.5mg / L+NAA 1.5mg / L+paclobutrazol 1.5mg / L, light intensity 2000Lux, light time 16 hours a day, temperature 28°C. After 25 days of root-promoting culture, it can be seen that the sugarcane tissue culture seedlings grow whiter and healthy roots.
[0041] 2) Pretreatment of rooted seedlings before transportation: take out the domesticated and harde...
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