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Wild grape VyGOLS gene and application of coding protein of wild grape VyGOLS gene to drought coercion

A wild grape, gene technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as lack of understanding of specific functions, and achieve the effect of enhancing drought resistance

Active Publication Date: 2019-11-15
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although, there are already some species in the prior art GOLS gene reports, but in wild grapes GOLS Genes have not been reported yet, people for wild grapes GOLS There is also a lack of understanding of the specific function of the gene

Method used

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  • Wild grape VyGOLS gene and application of coding protein of wild grape VyGOLS gene to drought coercion
  • Wild grape VyGOLS gene and application of coding protein of wild grape VyGOLS gene to drought coercion
  • Wild grape VyGOLS gene and application of coding protein of wild grape VyGOLS gene to drought coercion

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Embodiment 1

[0032] Wild grape in this embodiment VyGOLS Gene, the nucleotide sequence of which is shown in the 186-1196 positions in SEQ ID NO.1.

[0033] Example 1 of wild grape VyGOLS protein

[0034] The amino acid sequence of VyGOLS protein in this example is shown in SEQ ID NO.2.

[0035] Example 1 of recombinant expression vector

[0036] In this embodiment, the recombinant expression vector comprises wild grape VyGOLS gene, the wild grape VyGOLS The nucleotide sequence of the gene is shown as 186-1196 in SEQ ID NO.1.

[0037] Example 1 of the preparation method of recombinant expression vector

[0038]The preparation method of the recombinant expression vector in this embodiment includes: designing primers according to the sequence shown in positions 186-1196 in SEQ ID NO.1, and cloning the wild grape VyGOLS gene, and then the wild grape VyGOLS The gene is connected to the pCAMBIA2300 plant expression vector to obtain.

[0039] wild grapes VyGOLS Example 1 of the Applicati...

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Abstract

The invention relates to a wild grape VyGOLS gene and an application of coding protein of the wild grape VyGOLS gene to drought coercion, and belongs to the technical field of plant genetic engineering. A genetically modified technique of a strong promoter (cauliflower mosaic virus 35S promoter) driving principle is adopted, an overexpression vector of a V.yanshanensis VyGOLS gene is transferred to arabidopsis thaliana, and a genetically modified arabidopsis thaliana plant is obtained. Experiment verifies that compared with an arabidopsis thaliana plant for transforming an empty vector, the overexpression VyGOLS gene can cause the accumulation of stress tolerance relevant substances in the genetically modified arabidopsis thaliana and expression of drought tolerance related genes, and thegenetically modified plant is high in drought resistance. Therefore, the wild grape VyGOLS gene and the recombinant expression vector thereof can be used for breeding of plant drought-resistant varieties.

Description

technical field [0001] The present invention relates to wild grape VyGOLS The application of the gene and its coded protein in drought stress belongs to the technical field of plant genetic engineering. Background technique [0002] There are many kinds of grapes, which have important food value and economic value. Drought has a serious impact on the growth and development process, yield and quality of grapes, and has become one of the main factors restricting grape growth and improving fruit quality. Especially in recent years, global climate change and frequent droughts in southern my country have made grapes The industry is under serious threat. Under the background of water shortage in the world, it is of great scientific value and significance to discover drought-resistant grape resources and study grape drought-resistant genes to improve grape drought resistance, cultivate new drought-resistant varieties and water-saving cultivation. [0003] Drought resistance of gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00A01H6/20
CPCC12N9/1051C12Y204/01123C12N15/8273
Inventor 余义和郭大龙杨盛迪李旭飞孟祥轩杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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