Application of grape VlKNOX gene in promoting cytokinin synthesis and regulating fruit setting

A grape and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of lack of understanding of specific functions, etc., and achieve the effects of increasing fruit setting rate, increasing cytokinin content, and enhancing plant high-yield performance

Active Publication Date: 2019-11-15
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although, there are already some species in the prior art KNOX gene reports, but in grapes KNOX The gene has not been reported yet, people for the grape KNOX There is also a lack of understanding of the specific function of the gene

Method used

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  • Application of grape VlKNOX gene in promoting cytokinin synthesis and regulating fruit setting
  • Application of grape VlKNOX gene in promoting cytokinin synthesis and regulating fruit setting
  • Application of grape VlKNOX gene in promoting cytokinin synthesis and regulating fruit setting

Examples

Experimental program
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Embodiment 1

[0029] Grapes in this example wxya Gene, the nucleotide sequence of which is shown in the 181-1509 positions in SEQ ID NO.1.

[0030] Example 1 of Grape VlKNOX Protein

[0031] The amino acid sequence of grape VlKNOX protein in this embodiment is shown in SEQ ID NO.2.

[0032] Example 1 of recombinant expression vector

[0033] In this embodiment, the recombinant expression vector contains grape wxya gene, the grape wxya The nucleotide sequence of the gene is shown as 181-1509 in SEQ ID NO.1.

[0034] Example 1 of the preparation method of recombinant expression vector

[0035]The preparation method of the recombinant expression vector in this embodiment includes: designing primers according to the sequence shown in positions 181-1509 in SEQ ID NO.1, and cloning the grape wxya gene, and then the grape wxya The gene is connected to the pCAMBIA2300 plant expression vector to obtain.

[0036] Grape wxya Example 1 of the Application of Genes in Plant Variety Breeding

[...

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Abstract

The invention relates to an application of grape VlKNOX gene for promoting cytokinin synthesis and regulating fruit setting, and belongs to the technical field of plant genetic engineering. In the invention, a transgenic technology of a strong promoter (cauliflower mosaic virus 35S promoter) driving principle is utilized to transfer an excess-expression vector of a VlKNOX gene into Arabidopsis thaliana so as to obtain a transgenic Arabidopsis thaliana plant. Experiments prove that: compared with an Arabidopsis thaliana transformed with an empty vector, excessive expression of the VlKNOX gene leads to large accumulation of cytokinins in the transgenic Arabidopsis thaliana, and the fruit setting rate of fruits is obviously improved. Therefore, the grape VlKNOX gene and the recombinant expression vector thereof can be used for breeding high-yield plant variety.

Description

technical field [0001] The present invention relates to grapes wxya The application of gene promoting cytokinin synthesis to regulate fruit setting belongs to the technical field of plant genetic engineering. Background technique [0002] Grape is one of the important economic fruit trees widely cultivated in the world, and its fruit is mainly used for wine making, fresh food, juice making and drying. Grapes and wine are rich in minerals, vitamins and various essential amino acids that are beneficial to the human body. Among them, resveratrol has the effects of anti-cancer, anti-inflammation, anti-aging and improving immunity, which has attracted worldwide attention and promoted the development of the world's grape industry. American grapes or European and American grape hybrids have excellent fruit quality and strong stress resistance, and occupy a dominant position in the table grape industry. However, American grapes or European and American hybrid grapes show the char...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/66A01H5/00A01H6/20
CPCC07K14/415C12N15/8261
Inventor 余义和郭大龙杨盛迪李旭飞孟祥轩杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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