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Application of CBLB502 protein in prevention and treatment of post-traumatic stress disorder

1. CBLB502, post-traumatic stress technology, applied to peptide/protein components, medical preparations containing active ingredients, nervous system diseases, etc., can solve the problems of PTSD treatment bottlenecks, many adverse reactions, slow onset, etc.

Active Publication Date: 2019-11-08
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They all have defects such as slow onset of effect (2-6 weeks), low effective rate (50%), and many adverse reactions (cognitive impairment, sexual dysfunction).
At present, there is almost no specific target for anti-PTSD except 5-HT transporter, which makes the treatment of PTSD fall into a "bottleneck". Therefore, it is of pioneering significance to discover potential new pharmacological targets for anti-PTSD

Method used

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  • Application of CBLB502 protein in prevention and treatment of post-traumatic stress disorder
  • Application of CBLB502 protein in prevention and treatment of post-traumatic stress disorder
  • Application of CBLB502 protein in prevention and treatment of post-traumatic stress disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of CBLB502 protein

[0039] The CBLB502 protein is shown in sequence 2 of the sequence listing, and its coding gene is shown in sequence 1 of the sequence listing.

[0040] 1. Replace the fragment between the SmaI and SalI restriction sites of the plasmid pBV220 with the DNA molecule shown in Sequence 1 of the Sequence Listing to obtain a recombinant expression vector.

[0041] 2. Transform Escherichia coli BL21(DE3) with the recombinant expression vector obtained in step 1 to obtain recombinant bacteria.

[0042] 3. Inoculate the recombinant bacteria obtained in step 2 into LB medium (2×YT basal medium can also be used instead), shake culture at 30°C, 200rpm for 6h, induce at 42°C, 150-200rpm for 3 hours, and the induction is over Then centrifuge at 8000rpm for 15 minutes to collect the bacterial cells.

[0043] 4. Resuspend the cell pellet obtained in step 3 with purified water, put it on ice and ultrasonically disrupt it to obtain a wh...

Embodiment 2

[0046] Example 2. Application of CBLB502 protein in prevention and treatment of post-traumatic stress disorder.

[0047] 1. The experimental animals (C57BL / 6 mice) were divided into a control group, a control administration group, a model group and a model administration group, with 10 mice in each group.

[0048] 2. After step 1 is divided into groups, the experimental animals are placed in a conventional feeding environment for one week (the first week).

[0049] 3. After completing step 2, start grouping in the second week and perform the following operations:

[0050] Model group: In the second week, electrical stimulation was performed after intraperitoneal injection of 0.1 ml of PBS; the electrical stimulation method was as follows: the mice were placed in the electric shock box and allowed to explore freely for 5 minutes (spontaneous activity time), followed by 5 minutes (stimulation time). 15 inescapable foot strikes (0.8mA, interval 10s, duration 10s) were intermitte...

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PUM

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Abstract

The invention discloses an application of CBLB502 protein in prevention and treatment of post-traumatic stress disorder. The invention finds for the first time that CBLB502 can significantly inhibit symptoms of PTSD syndrome induced by electrical stimulation. The CBLB502 can be used for preventing and treating the PTSD. The invention is of great significance to the research of PTSD prevention andtreatment methods.

Description

technical field [0001] The invention relates to the field of prevention and treatment of post-traumatic stress disorder, in particular to the application of CBLB502 protein in the prevention and treatment of post-traumatic stress disorder. Background technique [0002] Post-traumatic stress disorder (PTSD) refers to the individual suffering from major, threatening, catastrophic traumatic events such as wars, terrorist attacks, major disasters (earthquakes, traffic accidents, etc.) or sexual assault. The incurable chronic persistent mental illness manifested, resulting in impairment of professional ability and social function, originated from the description of chronic mental illness in combat stress-related mental behaviors in war. With the huge destructive power and deterrent power of modern high-tech wars, as well as the huge pressure on soldiers' psychology caused by certain special field environments, it is easy to cause psychological and physiological stress regulation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P25/00
CPCA61K38/164A61P25/00
Inventor 葛常辉王晓辉付汉江郑晓飞
Owner ACADEMY OF MILITARY MEDICAL SCI
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