Method for Improving Color Sorting Accuracy of Rice Genetic Engineering Male Sterile Line Seeds by Utilizing Dominant Black Glue Trait

Abstract

The invention discloses a method for increasing color sorting precision of nucleic sterile line seeds of rice heredity engineering through dominant black glume traits. The method specifically comprises the steps of breeding yellow glume dual color sorting heredity engineering nucleic sterile line; transmitting four chain expression genes including a dominant black glume gene, a fertility restorergene, a pollen inactivation gene and a fluorescence labeling gene into a yellow glume dual color sorting heredity engineering nucleic sterile line genome for breeding a black glume dual color sortingheredity engineering propagation line; enabling the black glume dual color sorting heredity engineering propagation line and the yellow glume dual color sorting heredity engineering nucleic sterile line to be planted in a mixed manner, and collecting all seeds in a mixed manner; performing screening on the seeds collected in a mixed manner according to the color of glumes for the first time, and separating yellow glume sterile line seeds from black glume seeds; and performing screening for the second time according to whether endosperm emits fluorescence or not, and refining the yellow glume sterile line seeds. According to the method disclosed by the invention, the dominant black glumes of rice are used as a new color sorting marker, the purity of the heredity engineering nucleic sterileline seeds in the sorting process is increased, and the purpose of enabling the purity of the heredity engineering nucleic sterile line to reach the production and application standard is realized.

Description

technical field [0001] The invention relates to the technical field of rice genetic engineering, in particular to a method for improving the color sorting accuracy of rice genetically engineered male sterile line seeds by utilizing the dominant black glume trait. Background technique [0002] The breeding and sorting of genetically engineered male sterile lines is the core technology of the third-generation hybrid rice, and the batch breeding of genetically engineered sterile line seeds and endosperm fluorescence sorting technology have been realized (see patent ZL201210426678. The industrialization of engineering nuclear sterile lines laid the foundation. However, in the process of using the existing fluorescence sorting technology to separate the CMS seeds, because the rice endosperm is wrapped by the glume, the intensity of fluorescence emitted by the fluorescent protein from the endosperm to the outside of the glume is reduced, which interferes with the color sorting of ...

AI Technical Summary

Problems solved by technology

However, in the process of using the existing fluorescence sorting technology to separate the sterile line seeds, because the rice endosperm is wrapped by the glume, the intensity of fluorescence emitted by the fluorescent protein from the endosperm to the outside of the glume is reduced, which interferes with the color sorting of the color sorter. Accuracy, the genetically engineered male sterile line selected often contains a few seeds of the breeding line with genetically modified components

Since the public is very sensitive to genetically modified products and the country has not released restrictions on genetically modified products, if a few seeds of the reproductive line carrying genetically modified ingredients are mixed with the seeds of the sterile line during the seed production process, it will result in the production of hybrid seeds containing A small number of seeds carry genetically modified ingredients, which seriously hinders the promotion and application of third-generation hybrid rice

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