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Fluorescent probe for detecting butyrylcholinesterase and its application

A technology for detecting butyrylcholinesterase and butyrylcholine, which is applied in the field of fluorescent probes for detecting butyrylcholinesterase, and can solve the problems of low atom utilization, poor stability of polypeptide fluorescent probes, and storage and transportation conditions. Harsh and other issues

Active Publication Date: 2021-05-07
THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of fluorescent probe and its application for detecting butyrylcholinesterase, aim to solve the purpose of the present invention is to overcome the detection method of butyrylcholinesterase in the prior art need to rely on expensive Large-scale equipment and complex detection process, while polypeptide fluorescent probes have problems of poor stability, harsh storage and transportation conditions, low atom utilization rate, and weak fluorescence intensity

Method used

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  • Fluorescent probe for detecting butyrylcholinesterase and its application
  • Fluorescent probe for detecting butyrylcholinesterase and its application
  • Fluorescent probe for detecting butyrylcholinesterase and its application

Examples

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Embodiment 1

[0076] This example is used to illustrate the method for detecting the activity of butyrylcholinesterase by the compound represented by formula (1) provided by the present invention.

[0077] Dissolve 1 mg of butyrylcholinesterase sample in 100 mM Tris-HCl (containing 10 mM CaCl 2 , pH=7.0) buffer solution and carry out gradient dilution, prepare the butyrylcholinesterase standard solution of different concentrations according to the concentration given in table 1, the butyrylcholinesterase standard solution that configuration obtains is placed in Preheat in a constant temperature water bath at 30°C; at the same time, place a black flat-bottomed 96-well plate in a 30°C incubator to preheat. Subsequently, 40 μL of preheated butyrylcholinesterase standard solution was placed in a preheated black flat-bottomed 96-well plate, and then 120 μL of 100 mM Tris-HCl (containing 10 mM CaCl 2 , pH=7.0) buffer solution, and a negative control group (only 160 μl of buffer solution was adde...

Embodiment 2

[0082] This example is used to illustrate the method for measuring the activities of elastase, butyrylcholinesterase, trypsin and carboxypeptidase using the non-peptide fluorescent probe represented by formula (1).

[0083]

[0084] Each butyrylcholinesterase sample was dissolved in 100mM Tris-HCl (containing 10mM CaCl 2 , pH=7.0) buffer solution and diluted to prepare an enzyme solution with a concentration of 0.01 mg / L; at the same time, place a black flat-bottomed 96-well plate in a 30°C incubator to preheat. Subsequently, 40 μL of the preheated enzyme solution was placed in a preheated black flat-bottomed 96-well plate, and then 120 μL of 100 mM Tris-HCl (containing 10 mM CaCl 2 , pH=7.0) buffer solution, and a negative control group (only 160 μl of buffer solution was added), under light-shielding conditions, 40 μL of the probe with the structure shown in formula (1) was contacted with the enzyme solution and the control group respectively, and used The microplate rea...

Embodiment 3

[0092] This example is used to illustrate the screening method for butyrylcholinesterase inhibitors according to the present invention.

[0093] Dissolve 1 mg of butyrylcholinesterase sample in 100 mM Tris-HCl (containing 10 mM CaCl 2 , pH=7.0) in the buffer solution, the concentration of butyrylcholinesterase is 0.05mg / mL to be tested butyrylcholinesterase standard solution, the butyrylcholinesterase inhibitor tacrine is dissolved in 1mL 100mM Tris-HCl (containing 10mM CaCl 2 , pH=7.0) buffer solution to configure inhibitor solutions with concentration gradients of 0.1, 1, 4, 7, 10, and 20 μM, and dissolve 0.01 mmol of the fluorescent probe with the structure shown in formula (1) in 10 mL of 100 mM Tris-HCl (contains 10mM CaCl 2 , pH=7.0) buffer solution to be configured as a fluorescent probe solution with a concentration of 1 mM, and the butyrylcholinesterase standard solution, butyrylcholinesterase inhibitor tacrine solution and fluorescent probe The solution was prehea...

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Abstract

The present invention provides a fluorescent probe for detecting the activity of butyrylcholinesterase, the fluorescent probe is a non-peptide fluorescent probe, and the structure of the non-peptide fluorescent probe is shown in the following formula 1 :

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a fluorescent probe for detecting butyrylcholinesterase and its application. Background technique [0002] Butyrylcholinesterase (BChE) is an acetylcholine isoenzyme that exists in organisms. It is a non-specific esterase synthesized by the liver. Since the enzyme is synthesized in the liver and released into the plasma immediately, it is therefore , BChE activity in serum is a sensitive indicator of protein synthesis in hepatocytes. With the continuous improvement of detection technology and clinical diagnosis and treatment, butyrylcholinesterase is not only used to evaluate liver reserve function, but also extended to the detection and treatment of malignant tumors, nervous system diseases, cardiovascular diseases, metabolic syndrome and many other diseases. in the field of diagnosis. There are two main methods for the determination of butyrylcholinesterase activity. The first i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D277/66C09K11/06G01N21/64
CPCC07D277/66C09K11/06C09K2211/1007C09K2211/1037G01N21/6486
Inventor 潘雪华
Owner THE HONG KONG POLYTECHNIC UNIV SHENZHEN RES INST
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