Method for increasing weight of silk gland of bombyx mori

A technology of weight and silk, applied in the field of improving the weight of silk glands in silkworm, can solve problems such as lack of research, and achieve the effect of improving economic traits of silkworm

Active Publication Date: 2019-09-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bombyx mori lipokinetic hormone is one of silkworm neuropeptides, its main function is to regulate the dynamic balance and distribution of energy in silkworms, but there is a lack of research on silkworm neuropeptides regulating the growth of silk glands through energy distribution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] According to the sequence pQLTFTSGWGa, an amino acid protected with a 9-fluorenylmethyloxycarbonyl (Fmoc) group to the α-amino group is covalently linked to an insoluble solid phase carrier (p-alkoxy Benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to remove the Fmoc protective group on the α-amino group, and use 90% mass fraction of TFA solution to wash the amino acid-arm-resin, the next The pre-activated α-amino-protected amino acids are connected through a coupling reaction; after the reaction is completed, they are washed with 90% mass fraction of TFA solution, and then deprotection and coupling reactions are repeated until all amino acids are sequentially removed from the C-terminal ( The carboxyl end) is connected to the N end (amino end) to obtain the desired polypeptide; the ester bond between the peptide chain and the solid carrier is hydrolyzed with hydrogen fluoride (HF) to obtain the silkworm adipokinetic hormone A polypeptide sy...

Embodiment 2

[0035] According to the sequence pQLTFTSGWGa, an amino acid protected with a 9-fluorenylmethyloxycarbonyl (Fmoc) group to the α-amino group is covalently linked to an insoluble solid phase carrier (p-alkoxy Benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to remove the Fmoc protective group on the α-amino group, and use 90% mass fraction of TFA solution to wash the amino acid-arm-resin, the next The pre-activated α-amino-protected amino acids are connected through a coupling reaction; after the reaction is completed, they are washed with 90% mass fraction of TFA solution, and then deprotection and coupling reactions are repeated until all amino acids are sequentially removed from the C-terminal ( The carboxyl end) is connected to the N end (amino end) to obtain the desired polypeptide; the ester bond between the peptide chain and the solid carrier is hydrolyzed with hydrogen fluoride (HF) to obtain the silkworm adipokinetic hormone A polypeptide sy...

Embodiment 3

[0040] According to the sequence pQLTFTSGWGa, an amino acid protected with a 9-fluorenylmethyloxycarbonyl (Fmoc) group to the α-amino group is covalently linked to an insoluble solid phase carrier (p-alkoxy Benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to remove the Fmoc protective group on the α-amino group, and use 90% mass fraction of TFA solution to wash the amino acid-arm-resin, the next The pre-activated α-amino-protected amino acids are connected through a coupling reaction; after the reaction is completed, they are washed with 90% mass fraction of TFA solution, and then deprotection and coupling reactions are repeated until all amino acids are sequentially removed from the C-terminal ( The carboxyl end) is connected to the N end (amino end) to obtain the target polypeptide; the ester bond between the peptide chain and the solid carrier is hydrolyzed with hydrogen fluoride (HF) to obtain the silkworm adipokinetic hormone A polypeptide syn...

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Abstract

The invention discloses a method for increasing the weight of silk gland of bombyx mori. According to the sequence pQLTFTSGWGa, bombyx mori adipokinetic hormone A polypeptide synthesis is carried out from a C terminal (carboxyl terminal) to an N terminal (amino terminal); the activity of synthesized bombyx mori adipokinetic hormone A polypeptides is detected by adopting a luciferase reporter gene method; a bombyx mori adipokinetic hormone A active polypeptide solution is degermed by a filtering membrane, and injected into the abdomen of the 5-day bombyx mori in the second day to the third day, the bombyx mori is raised with mulberry leaves to be mature, so that the weight of the silk gland is increased. The method achieves the increase of the weight of the silk gland of the bombyx mori by regulating the distribution of bombyx mori energy by newly discovered active polypeptides without increasing the mulberry leaf eating quantity of the bombyx mori, which has a positive effect on the study of the energy distribution mechanism of the bombyx mori, further improvement of economic characters of the bombyx mori and the like.

Description

Technical field [0001] The invention relates to the synthesis of an active polypeptide for increasing the weight of the silk gland of the silkworm, in particular to a method for increasing the weight of the silk gland of the silkworm. Background technique [0002] Bombyx mori (Bombyx mori) is the largest amount of silk-secreting insects raised indoors on earth today. It takes in various nutrients from mulberry leaves and supplies various tissues and organs in the silkworm through metabolic physiological processes for growth, development, and reproduction. Supply nutrients to silk glands for silk gland growth. The silk gland is the organ of the silkworm that synthesizes silk material, and its growth directly affects the amount of silkworm silk. For this reason, adopting various methods to promote the growth of silk glands is one of the important technical measures to increase the yield of cocoon silk in sericulture, and it has always been widely valued. [0003] The content of neu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04C07K7/06C07K1/04C07K1/06
CPCA01K67/04C07K7/06
Inventor 时连根沈张飞屠振力
Owner ZHEJIANG UNIV
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