A strain of Pichia monteritima that degrades polyethylene and produces alkanes
A polyethylene, yeast technology, applied in the direction of microbe-based methods, microbes, microbes, etc.
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[0015] Specific embodiment 1: Pichia pastoris MeyerozymaguilliermondiiZJC-1, which can degrade polyethylene in this embodiment, is preserved in the General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, and the preservation address is No. 1, Beichen Road, Chaoyang District, Beijing Court No. 3, the deposit date is December 17, 2018, and the deposit number is CGMCC No: 16956.
[0016] In this embodiment, Pichia guilliermondii ZJC-1 is obtained by screening from the intestinal fluid of the larvae of the mellonella mellonella, and is identified according to the following method.
[0017] Molecular biological identification of the screened strains: Inoculate Meyerozyma guilliermondii ZJC-1 in YPD liquid medium, culture in a constant temperature shaking incubator at 30°C at 120r / min for 16-24h, take 2mL of the bacterial liquid and centrifuge at 8000rpm to collect the bacterial cells , Genomic DNA was extracted according to the OMEGA HP Funga...
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