Electrochemical fingerprint spectrum method for detecting edible bird's nests
A technology of fingerprint and detection method, which is applied in the field of electrochemical fingerprint detection of bird's nest, can solve the problems of identifying bird's nest, and achieve the effect of low cost and good application prospect
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Embodiment 1
[0028] Embodiment 1 Electrochemical fingerprint of the present invention identifies bird's nest
[0029] 1) Preparation of sample hydrolyzate
[0030] The sample to be tested was dried to constant weight, ground, and passed through a 120-mesh sieve. Add 0.1120g of sample powder and 5mL of 1.0mol / L sulfuric acid to the glass test tube, place it in an oven for hydrolysis at 40°C for 8 hours, filter, and take the filtrate to obtain the sample hydrolyzate;
[0031] 2) B-Z electrochemical oscillation fingerprint
[0032] Take step 1) 5mL sample hydrolyzate, 5mL 2.0mol / L H 2 SO 4 , 10mL 1.8mol / L HOOCCH 2 COOH, 10mL 0.016mol / L (NH 4 ) 4 Ce(SO 4 ) 4 , placed in a 150mL glass reaction vessel, shake well, and cover the reactor cover with injection holes and electrodes. At 35°C, with a stirring speed of 500r / min, add 10mL of 1.0mol / L KBrO 3 , at this time in the mixed solution [H + ]=0.75mol L -1 ,
[0033] [Ce 4+ ]=0.004mol L -1 、[HOOCCH 2 COOH]=0.45mol L -1 , [BrO 3 ...
Embodiment 2
[0035] Embodiment 2 Electrochemical fingerprint of the present invention identifies bird's nest
[0036] 1) Preparation of reference hydrolyzate
[0037] Take the genuine bird's nest and dry it to constant weight, grind it, and pass it through a 120-mesh sieve. Add 0.1120g of bird’s nest powder and 5mL of 1.0mol / L sulfuric acid to the glass test tube, place in an oven for hydrolysis at 40°C for 8 hours, filter, and take the filtrate to obtain bird’s nest hydrolyzate;
[0038] 2) Preparation of sample hydrolyzate
[0039] The sample to be tested was dried to constant weight, ground, and passed through a 120-mesh sieve. Add 0.1120g of sample powder and 5mL of 1.0mol / L sulfuric acid to the glass test tube, place it in an oven for hydrolysis at 40°C for 8 hours, filter, and take the filtrate to obtain the sample hydrolyzate;
[0040] 3) B-Z electrochemical oscillation fingerprint
[0041] Step 1) 5mL bird's nest hydrolyzate, 5mL 2.0mol / L H 2 SO 4 , 10mL 1.8mol / L HOOCCH 2 CO...
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