Method for increasing production of methane through corn straw anaerobic dry fermentation bacteria agent and biogas liquid reflux

A technology for corn stalks and fermentation inoculants is applied in the field of corn stalk anaerobic dry fermentation inoculants and biogas slurry reflux to increase methane production. The effect of liquid production, promotion of fermentation gas production, and stable heat release

Pending Publication Date: 2019-08-30
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI +2
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  • Description
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Problems solved by technology

[0005] The above-mentioned technologies all adopt the conventional methane digester fermentation method to produce biogas. Due to the low concentration of raw materials and high water content in the fermentation process, problems such as slow gas production speed, low raw material decomposition rate, and low gas production rate are caused.
However, if the concentration of raw materials is increased, the excessive decomposition speed of microorganisms will lead to local acidification of the fermentation system, excessive temperature and other problems, resulting in the collapse of the fermentation system and the failure to realize the normal fermentation process.

Method used

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  • Method for increasing production of methane through corn straw anaerobic dry fermentation bacteria agent and biogas liquid reflux
  • Method for increasing production of methane through corn straw anaerobic dry fermentation bacteria agent and biogas liquid reflux
  • Method for increasing production of methane through corn straw anaerobic dry fermentation bacteria agent and biogas liquid reflux

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Such as Figure 1-3 The device for increasing the production of methane by returning biogas slurry from anaerobic dry fermentation of corn stalks includes:

[0045] Anaerobic dry fermentation tank 1, with a volume of 300m 3 The airtight tank body is provided with a feed hole 3 and a liquid inlet hole 4 on the tank body, an air outlet hole 2 is provided on the top of the tank body, the bottom of the tank body is inclined at 3-5°, and the bottom end of the anaerobic dry fermentation tank 1 is provided with a Biogas slurry outlet 5, which is convenient for biogas slurry collection to flow into biogas slurry outlet 5;

[0046] Biogas slurry storage tank 6, with a volume of 3m 3 The airtight liquid storage tank is provided with a storage tank liquid inlet and a storage tank liquid outlet, the storage tank liquid inlet is connected with the biogas slurry outlet 5 of the anaerobic dry fermentation tank 1 through a pipeline 7, and the storage tank liquid outlet is connected t...

Embodiment 2

[0051] A corn stalk anaerobic dry fermentation bacterial agent prepared by the following method:

[0052] (1) Get Bacillus pumilus, through activation, seed culture, expansion culture, the obtained thalline concentration is 10×10 9 The Bacillus pumilus fermentation liquid of cfu / g;

[0053] (2) get bacillus halotolerant, through activation, seed culture, expanded culture, the obtained thalline concentration is 6 * 10 9 The halotolerant bacillus fermentation liquid of cfu / g;

[0054] (3) Get the jelly-like bacillus, through activation, seed culture, expansion culture, the obtained thalline concentration is 6 * 10 9 The jelly-like bacillus fermentation liquid of cfu / g;

[0055] (4) Get Paenibacillus polymyxa, through activation, seed culture, and expanded culture, the obtained thalline concentration is 6×10 9 Paenibacillus polymyxa fermentation broth of cfu / g;

[0056] (5) Get Enterococcus faecalis, through activation, seed culture, expansion culture, the obtained bacterium...

Embodiment 3

[0067] Using the different microbial agents prepared in Example 2 and Comparative Examples 1-2 respectively, combined with the device in Example 1, methane production is carried out according to the following method, and the specific steps are as follows:

[0068] (1) Crushing the corn stalks with a pulverizer to a particle size of 1.5 cm to obtain straw chips;

[0069] (2) adding water to the straw chips obtained in step (1) to adjust the water content to 70%, putting them into an anaerobic dry fermentation tank 1, and performing anaerobic fermentation for 2 months to obtain pre-fermentation raw materials;

[0070] (3) add biogas residues of equal quality to the pre-fermentation raw material of step (2) in the anaerobic dry fermentation tank 1 through the feed hole 3, then add the fermenting bacterial agent of 4% of the pre-fermentation raw material quality, at a temperature of 36 ° C, Under the condition of pH value 8.0, anaerobic dry fermentation is carried out, and the bio...

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Abstract

The invention relates to a method for increasing production of methane through a corn straw anaerobic dry fermentation bacteria agent and biogas liquid reflux. The corn straw anaerobic dry fermentingagent is prepared from the following effective microbial components: bacillus pumilus of (8-10)*10<9> cfu / g, bacillus halodurans of (4-6)*10<9> cfu / g, bacillus mucilaginosus of (4-6)*10<9> cfu / g, bacillus polymyxa of (4-6)*10<9> cfu / g and enterococcus faecalis of (2-4)*10<9> cfu / g. Biogas is prepared by a corn straw dry fermentation method for the first time and the biogas liquid is sprayed abovethe fermented materials through biogas liquid reflux, so that the problems that a fermentation system is liable to acidify and the local temperature is too high caused by high material concentration are solved and the condition that the fermentation system breaks down is avoided.

Description

technical field [0001] The invention relates to a corn stalk anaerobic dry fermentation bacterial agent and a method for increasing methane production by recirculating biogas slurry, and belongs to the technical field of biological fermentation. Background technique [0002] Corn is a crop that is mainly used as feed for grain, economics, and feed. Corn stalks, as an agricultural waste rich in nutrients and usable components, are also important resources for industrial and agricultural production. Every year, more than 700 million tons of various straws are produced in my country's agricultural production, and corn straws account for more than 50%. How to turn waste into treasure and utilize corn and other crop stalks with high efficiency and low cost has become a research and development hotspot in the field of biomass resource utilization in countries around the world. However, due to the excessive production of corn stalks, the geographical dispersion of the output, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P5/02C12R1/07C12R1/12C12R1/01
CPCC12N1/20C12P5/023Y02E50/30
Inventor 王艳芹左斌付龙云仲子文张元成王云鹤雷军
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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