Cell or Particle Counting Method Based on Surface Acoustic Wave Technology

Abstract

The invention discloses a cell or particle counting method based on surface acoustic wave technology. Existing cell counting methods are either only suitable for large volume cell counts or are expensive or take too long. The present invention makes a microfluidic channel on the surface acoustic wave propagation path of a surface acoustic wave device; connects a syringe with a micropump to transport cell fluid or particle fluid into the microfluidic channel, uses sheath flow technology to drive the cell fluid or particle fluid to achieve focusing, and makes the microfluidic The cells or particles in the flow channel are in a single arrangement, and the distance between the cells or particles is kept, and they flow through the micro-flow channel in turn; the surface acoustic wave device is connected to the detection instrument, and after the detection instrument works, the transmission parameters are measured. By drawing the transmission parameters at the resonance frequency An image of point values ​​over time to obtain cell or particle population information. The present invention realizes by testing transmission parameters, achieves real-time test data, and improves technical precision.

Description

technical field [0001] The invention relates to cell counting technology, in particular to a cell or particle counting method based on surface acoustic wave technology. Background technique [0002] Cells are the most basic units that constitute the structure and function of living organisms and perform various functions of living organisms. Every day in the human body, many cells (RBCs) age and die and many cells are regenerated to maintain the dynamic balance of the metabolism of the living body. But if this homeostasis is disrupted, it will cause a series of health problems. By detecting the number of specific cells, certain diseases can be identified and diagnosed, so cell counting is of great significance to health care, disease treatment, and medical research, and it is also a very useful tool. For example, CD+4 lymphocytes in HIV-infected people will gradually decrease, and detection of the number of CD+4 lymphocytes can help diagnose the disease; red blood cells in...

AI Technical Summary

Problems solved by technology

Although this method has fast detection speed and high detection accuracy, the supporting equipment is expensive, the operation is complicated, the detection cost is high, and the operating environment requirements are high. It is difficult to popularize in developing countries or remote areas, and its function is not only for cells. count a case

This will be somewhat wasteful for detection experiments that only have specific requirements

[0006] Image cytometer technology: a new type of cell detection technology combined with microscope and flow cytometry, combining optical precision instrument measurement of cell morphological parameters with image processing technology, not only can measure a large number of cells, but also can study the morphology of specific cells, and obtain cell follow-up Time-varying information and morphological characteristics of cells inside the tissue, but this imaging method takes a long time and cannot obtain the desired results in real time

[0007] In view of the above-mentioned disclosed cell counting method, there are several defects. Although red blood cell counting can quickly obtain the result, the result may be high, and it is only suitable for large-volume cell counting; flow cytometry has fast detection speed and high accuracy. However, the price is expensive, and it is difficult to popularize in remote areas; the imaging time of image cytometer is too long, and the results cannot be obtained in real time

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