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Detection reagent for detecting anti-hepatoma natural antibodies, reagent kit and method

A technology for detecting reagents and natural antibodies, applied in the field of immunology

Active Publication Date: 2019-08-16
QINGDAO HAILANSHEN BIOTECH
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AI Technical Summary

Problems solved by technology

It is speculated that the risk of developing tumors in those who lack or are negative for natural antibodies may be significantly higher than that in the normal population

Method used

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  • Detection reagent for detecting anti-hepatoma natural antibodies, reagent kit and method
  • Detection reagent for detecting anti-hepatoma natural antibodies, reagent kit and method
  • Detection reagent for detecting anti-hepatoma natural antibodies, reagent kit and method

Examples

Experimental program
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Embodiment Construction

[0058] 1. Screen healthy human plasma rich in natural antibodies against liver cancer

[0059] According to the "Blood Donation Law of the People's Republic of China" and the relevant regulations of the national health administrative department, each blood donor can voluntarily donate about 300 ml of blood each time, and 150-200 ml of plasma can be obtained by processing under sterile conditions. The levels of natural antibodies rich in anti-liver cancer were detected in the plasma samples of healthy blood donors provided by a blood station in Shandong Province. The two polypeptide antigens (see Table 3) used in this experiment were synthesized by chemical solid phase with a purity of 95%. Specifically, perform the following steps:

[0060] (1) Before operation, the two antigenic polypeptides derived from VEGFR1 and FGFR2 shown in Table 3 were dissolved in 67% acetic acid into 5.7 mg / ml stock solutions respectively, then mixed in equal volumes, and stored in a -20°C refrigera...

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Abstract

The invention relates to an antigen polypeptide detection reagent used for detecting VEGFR1 (vascular endothelial growth factor receptor 1) and FGFR2 (fibroblast growth factor receptor 2) natural antibodies, a reagent kit containing the detection reagent, a method for detecting the concentrations of the VEGFR1 and FGFR2 natural antibodies in a sample by use of the detection reagent or the reagentkit as well as an application of the detection reagent or the reagent kit in detection of the VEGFR1 and FGFR2 natural antibodies in the sample. Two linear antigen polypeptides completely complementary with the VEGFR1 and FGFR2 natural antibodies are used for qualitative and quantitative detection of the anti-hepatoma natural antibodies in the sample and the detection reagent and the reagent kit can be used for distinguishing blood plasma containing rich VEGFR1 and FGFR2 natural antibodies and blood plasma without VEGFR1 and FGFR2 natural antibodies.

Description

technical field [0001] The invention belongs to the technical field of immunology and relates to two peptide antigens, which can be applied to the preparation of enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) antibody detection kits for detecting the concentration of anti-hepatoma natural antibodies in human plasma. Background technique [0002] Primary liver cancer (mainly hepatocellular carcinoma, Hepatocellular Carcinoma, HCC) is the fifth most common malignant tumor in the world and the third leading cause of death from all tumors. Its 5-year survival rate is less than 10%. According to reports, there are nearly 800,000 new cases of liver cancer every year in the world, of which more than 400,000 are in China. The pathogenesis of most patients is closely related to infection with hepatitis B or C. The mortality rate of primary liver cancer among cancer patients in China ranks second in males and third in females. Although there are many tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6854G01N33/6893G01N33/57438
Inventor 韩传伟王明民
Owner QINGDAO HAILANSHEN BIOTECH
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