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An application of 05sar-page

A protein and solution technology, applied in the field of 05SAR-PAGE and its preparation, can solve the problems of destruction, no enzyme activity analysis, etc., and achieve the effect of simple and convenient operation

Active Publication Date: 2020-08-28
WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have shown that 0.1% w / v SAR will destroy the structure of some conventional proteins, so SAR-PAGE containing 0.1% w / v SAR is not used for the analysis of protein aggregation status and protein enzyme activity

Method used

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  • An application of 05sar-page
  • An application of 05sar-page
  • An application of 05sar-page

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0058] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0059] 1. 05SAR-PAGE

[0060] Table 1 prepares the solution used for Tris-glycine 6% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0061]

[0062] Table 2 prepares the solution used for Tris-glycine 8% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0063]

[0064] Table 3 prepares the solution used for Tris-glycine 10% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0065]

[0066]

[0067] Table 4 prepares the solution used for Tris-glycine 12% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0068]

[0069] Table 5 prepares the solution used for Tris-glycine 15% 05SAR-PAGE polyacrylamide gel electrophoresis separation gel

[0070]

[0071] Table 6 prepares the solution used for Tris-glycine 05SAR-PAGE polyacrylamide gel electrophoresis stacking gel

[0072]

[0073] 2. Application of 05SAR-PAGE

[0...

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Abstract

Provided are a 05SAR-PAGE, a preparation method therefor, and an application thereof, relating to a gel electrophoresis separation technique. The 05SAR-PAGE is a gel prepared from sodium lauroyl sarcosinate with a mass-volume ratio of 0.05%, and comprises a lower separation gel and an upper concentration gel. The lengths and thicknesses of the lower separation gel and the upper concentration gel are the same, and the ratio of the height of the lower separation gel to the height of the upper concentration gel is equal to 2:1 to 8:1. The present invention resolves the shortcomings in which SDS-PAGE cannot be used to analyze physiological state protein modification and native-PAGE cannot use protein markers for calibration and cannot be directly applied to the analysis of acidic and basic proteins. The present invention has universality, simple operation, and low costs, and is widely applicable to fundamental and applied research in the fields of molecular dynamics, enzymology, medicine, etc., bringing extensive convenience to the scientific analysis of proteins for experimenters.

Description

technical field [0001] The invention relates to a gel electrophoresis separation technology, in particular to a 05SAR-PAGE and its preparation method and application. Background technique [0002] Protein is an important component of organisms and the main bearer of life activities. Proteins often exist in multimeric state, complex state or modified state to perform biological functions. At present, it is still challenging to accurately identify different states of proteins and protein interactions. [0003] SDS-PAGE and Native-PAGE are the most commonly used protein identification methods in the field of protein research. SDS is a strong anionic detergent, which can bind to proteins and break the structure of spherical proteins, so that the molecular weight of proteins is related to the amount of charge carried by proteins, so the migration speed of proteins during electrophoresis depends only on the molecular weight of proteins , and the protein marker can be used to ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N33/68B01D57/02
CPCB01D57/02G01N27/447G01N33/6803
Inventor 黄丽群姜凌李从刚刘乙祥郑稳稳
Owner WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
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