A whole blood microfluidic automatic cross matching system and blood matching method

Abstract

The invention provides a whole blood microfluidic automatic cross-matching system, which includes a blood matching card, an incubation device connected to the sample outlet of the blood matching card, and a detection device connected to the sample outlet of the incubation device. The incubation device includes the first The first mixed incubation area and the second mixed incubation area, the blood matching card includes the diluent quantitative distribution system, the blood supply quantitative distribution system and the blood receiving quantitative distribution system, the sample outlet of the serum quantitative distribution system in the blood supply quantitative distribution system and the blood receiving quantitative distribution The sample outlets of the red blood cell quantitative area in the system are all connected with the first mixed incubation area, the sample outlets of the red blood cell quantitative area in the blood supply quantitative distribution system and the sample outlets of the serum quantitative area in the blood receiving quantitative distribution system are connected with the first mixed incubation area. The two mixing incubation areas are connected. The invention realizes the automatic separation, quantification, mixing and detection process of serum and red blood cells. It is easy to operate and realizes the integration and automation of cross-matching blood detection. At the same time, the invention also provides a whole blood microflow Control the blood matching method of the automatic cross matching system.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a whole blood microfluidic automatic cross-matching system and a blood-matching method. Background technique [0002] With the continuous development of clinical medicine, blood transfusion has become a necessary means of clinical rescue and treatment. However, in the clinical process of blood transfusion, adverse reactions occur from time to time, and these adverse reactions usually have serious consequences or even life-threatening. Therefore, it is an important prerequisite and guarantee for safe blood transfusion to conduct a cross-matching test before clinical blood transfusion to check the compatibility between the blood recipient's red blood cell antigen and the antibody in the serum and the blood donor. At present, the test methods commonly used for cross-matching in clinical medicine mainly include saline method, polybrene method, and antihuman glo...

AI Technical Summary

Problems solved by technology

The process of liquid preparation and sample addition is cumbersome, and it is easy to be confused during manual operation. For example, when adding samples, the red blood cell suspension does not fully contact with the serum sample or part of it enters the small column tube below, which may cause incomplete incubation and affect the test results. Put the card vertically on the incubator to incubate, and then put it on a special centrifuge for horizontal centrifugation. After centrifugation, return the reagent card to the vertical direction and use the camera system or the naked eye to interpret the results. The reagent card is on multiple operating platforms and in two directions. Frequent changes, complex operation

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