A method for preparing Shi's minerals based on ZVI reduction coupled microorganisms

A technology of Shi's minerals and microorganisms, applied in the field of environmental engineering, can solve the problems of low amount of synthetic minerals in shake flask batches, low total iron precipitation rate, low iron precipitation rate and low synthetic minerals, and achieve the goal of avoiding a lot of manpower and material resources Effects of consumption, increase of mineral synthesis, and improvement of total iron precipitation rate

Active Publication Date: 2021-10-08
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, regardless of the chemical method or the biological method, the iron precipitation rate and the amount of synthetic minerals when the final stability is reached during the synthesis process using the shake flask method in the laboratory are relatively low, which can only reach 43.1%, 36.7% and 4g respectively. / L, 3.2g / L, with a lot of Fe 3+ Mineral formation has not been utilized, and there are defects of low total iron precipitation rate and low amount of synthetic minerals in shake flask batches
[0004] As mentioned above, the environmental significance of Shi's minerals is very obvious, but there is no 3+ A Continuous Production Method Related to Utilization Rate and Synthetic Efficiency of Schwartz Minerals

Method used

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  • A method for preparing Shi's minerals based on ZVI reduction coupled microorganisms
  • A method for preparing Shi's minerals based on ZVI reduction coupled microorganisms
  • A method for preparing Shi's minerals based on ZVI reduction coupled microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Prepare Shi's mineral under the condition of HRT=1.5 days:

[0045] The purchased A.ferrooxidans LX5 (Chinese patent: 02112924.X) was inoculated in the improved 9K medium (composition: (NH 4 ) 2 SO 4 3.5g / L, KCI 0.119g / L, K 2 HPO 4 0.058g / L (or K 2 HPO 4 ·3H 2 (00.076g / L), MgSO 4 ·7H 2 O 0.583g / L, Ca(NO 3 ) 2 4H 2 O 0.0168g / L, energy substance FeSO 4 ·7H 2 044.2g / L), use 1:1H 2 SO 4 Adjust the pH to 2.5, keep the total volume of the system at 3 L, place in a reciprocating shaker with a rotation speed of 180 r / min, and a temperature of 28° C. for 3 days. Place the bacterium solution after 3 days of expansion in container 1 (what this example adopts is a 3L beaker), add 22.24g / L FeSO 4 ·7H 2 0 and stir until it is completely dissolved, then continue to aerate and oxidize under the condition that the dissolved oxygen concentration is not lower than 6mg / L. When Fe in container 1 2+ After being fully oxidized by aeration, the mixed solution was continuou...

Embodiment 2

[0047] Prepare Shi's mineral under the condition of HRT=3 days:

[0048] A.ferrooxidans LX5 was inoculated in the improved 9K medium with 10% ratio (composition: (NH 4 ) 2 SO 4 3.5g / L, KCI 0.119g / L, K 2 HPO 4 0.058g / L (or K 2 HPO 4 ·3H 2 O 0.076g / L), MgSO 4 ·7H 2 O0.583g / L, Ca(NO 3 ) 2 4H 2 O 0.0168g / L, energy substance FeSO 4 ·7H 2 0 44.2g / L), with 1:1H 2 SO 4 Adjust the pH to 2.5, keep the total volume of the system at 3 L, place in a reciprocating shaker with a rotation speed of 180 r / min, and a temperature of 28° C. for 3 days. Place the bacterium solution after 3 days of expansion in container 1 (what this example adopts is a 3L beaker), add 22.24g / L FeSO 4 ·7H 2 0 and stir until it is completely dissolved, then continue to aerate and oxidize under the condition that the dissolved oxygen concentration is not lower than 6mg / L. When Fe in container 1 2+ After being fully oxidized by aeration, the mixed solution was continuously flowed into another contai...

Embodiment 3

[0050] Prepare Shi's mineral under the condition of HRT=4.5 days:

[0051] A.ferrooxidans LX5 was inoculated in the improved 9K medium with 10% ratio (composition: (NH 4 ) 2 SO 4 3.5g / L, KCI 0.119g / L, K 2 HPO 4 0.058g / L (or K 2 HPO 4 ·3H 2 O 0.076g / L), MgSO 4 ·7H 2 O0.583g / L, Ca(NO 3 ) 2 4H 2 O 0.0168g / L, energy substance FeSO 4 ·7H 2 0 44.2g / L), with 1:1H 2 SO 4 Adjust the pH to 2.5, keep the total volume of the system at 3 L, place in a reciprocating shaker with a rotation speed of 180 r / min, and a temperature of 28° C. for 3 days. Place the bacterial solution after 3 days of expansion into container 1, add 22.24g / L FeSO 4 ·7H 2 0 and stir until it is completely dissolved, then continue to aerate and oxidize under the condition that the dissolved oxygen concentration is not lower than 6mg / L. When Fe in container 1 2+ After being fully oxidized by aeration, the mixed solution was continuously flowed into another container 2 at a flow rate of 0.46mL / min, an...

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Abstract

The invention discloses a method for preparing Shi's minerals based on ZVI reduction coupled microorganisms, which comprises the following steps: (1) the bacteria liquid obtained by bacterial strain culture and FeSO 4 ·7H 2 0 mixed, stirred and dissolved to obtain the mixed solution; (2) the mixed solution obtained in step (1) was aerated and oxygenated in container 1 to Fe 2+ fully oxidized to Fe 3+ , transfer part of the mixed solution to another container 2, and add ZVI to mix and stir for Fe 3+ Reduction; (3) Fe in step (2) 3+ After reducing for a certain period of time, the solid-liquid separation ZVI and the reduced mixed solution; the separated mixed solution is transferred back to the container 1 for aeration and oxygenation and oxidation; (4) circulate according to steps (2) and (3), until the whole The total iron TFe in the system is completely precipitated, and the synthesized minerals in container 1 are collected by filtration, washed with water and dried to obtain the Schwartz minerals. This method not only improves the iron utilization rate and the total iron precipitation rate, but also continuously produces Shishi minerals.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and in particular relates to a method for efficiently preparing Schwittmannite minerals based on a zero-valent iron (ZVI) reduction coupling biological method. Background technique [0002] Schwittmannite mineral (referred to as Schweeter's mineral) is a secondary hydroxysulfate ferric mineral with strong surface group activity and special morphology. It is widely found in acidic mine wastewater and acidic sulfate soil. in an acidic environment. Shi's minerals have a strong adsorption capacity for toxic and harmful substances such as heavy metals and polycyclic aromatic hydrocarbons in the environment. The adsorption capacity of biosynthesized Shi's minerals to As(III) was as high as 113.9mg / g, and the maximum adsorption capacity to Cr(VI) was as high as 55mg / g, and the competing anions and ionic strength had little effect on the adsorption efficiency. Compared with chemical sedimentati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12P3/00C02F101/34C02F101/38C02F101/20C02F101/30
Inventor 郑冠宇王电站周立祥黄旭旦
Owner NANJING AGRICULTURAL UNIVERSITY
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