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Artificial breeding method of piliferocallus hezhangensis yang et zhan,sp.n.

A technology of artificial breeding and golden bee, which is applied in botany equipment and methods, horticulture, plant protection, etc., can solve the problems of Huashan pine withering, Huashan pine does not have secondary germination of needles and shoots, and achieves reproductive technology Mature, lower cost of artificial propagation, easy to obtain effect

Active Publication Date: 2019-06-14
ZUNYI INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Huashan pine does not have the characteristics of secondary germination of needles and shoots, and has no leaf sheath, when more than 90% of the needles are eaten, the whole Huashan pine will die

Method used

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  • Artificial breeding method of piliferocallus hezhangensis yang et zhan,sp.n.
  • Artificial breeding method of piliferocallus hezhangensis yang et zhan,sp.n.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Test method:

[0024] (1) Clean and sterilize the finger tube and dry it;

[0025] (2) using the larvae of Apis mellifera as a substitute host, and putting the substitute host into the bottom of the finger tube;

[0026] (3) Insert the fertilized A. chrysalis female wasp into the finger tube, the ratio of the number of the female A. chrysalis callus to the alternative host is 1:1, and the bee Plug the open end of the finger-shaped tube with absorbent cotton after the end, and put the finger-shaped tube into the artificial climate box to cultivate for 22-28 days, until all the emergence of the Herzpinus chrysalis callosus; the culture condition in the artificial climate box is : relative humidity 50%, temperature 25°C, photoperiod L12:D12. Observe the egg laying, hatching, larvae, and emergence of the Herzhang sawfly wasp, larvae, and eclosion every day until all eclosions emerge, and count the number of adult bees;

[0027] (4) Set up a control, replace the larvae...

Embodiment 2

[0032] 1. Test method:

[0033] (1) Clean and sterilize the finger tube and dry it;

[0034] (2) the larvae of the capping of Vespa aureus are used as a substitute host, and the substitute host is put into the bottom of the finger tube;

[0035] (3) Insert the fertilized A. chrysalis female wasp into the finger tube, the ratio of the number of the female A. chrysalis callus to the alternative host is 1:2, and the bee Plug the open end of the finger-shaped tube with absorbent cotton after the end, and put the finger-shaped tube into the artificial climate box to cultivate for 22-28 days, until all the emergence of the Herzhangus chrysalis callosus; the culture condition in the artificial climate box is : relative humidity 60%, temperature 25°C, photoperiod L10:D14. Observe the egg laying, hatching, larvae, and emergence of the Herzhang sawfly wasp, larvae, and eclosion every day until all eclosions emerge, and count the number of adult bees;

[0036] (4) Control is set, and ...

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Abstract

The invention discloses an artificial breeding method of piliferocallus hezhangensis yang et zhan,sp.n.. The method comprises the following steps of selecting larvae or pupae of bees or wasps as substitute hosts of piliferocallus hezhangensis yang et zhan,sp.n., screening out proper breeding conditions, and successfully achieving artificial large-scale breeding of piliferocallus hezhangensis yanget zhan,sp.n..

Description

technical field [0001] The invention relates to the technical field of biological control of pests, in particular to an artificial breeding method for golden hornets. Background technique [0002] Neodiprion huizeensis belongs to Diprionidae of Hymenoptera, Hymenoptera, and is a defoliator that seriously harms Huashan pine and Yunnan pine. In Guizhou, it is mainly distributed in Bijie, Weining and Hezhang, two counties where Huashan pine is concentrated and has a large area. The new pine sawfly in Huize has two generations in one year in Guizhou. It mainly cocoons and overwinters as pupae in the soil layer around the tree crown, under the stones and litter. The whole branch can be eaten up in a short time, and all the pine needles can be eaten up when the damage is serious. Because Huashan pine does not have the characteristics of secondary germination of needles and shoots, and has no leaf sheaths, when more than 90% of the needles are eaten, the entire Huashan pine will ...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G13/00
Inventor 展茂魁陈华赵斌司徒春南路纪芳方维业杨朝应杜文先孙亚茹王淑君
Owner ZUNYI INST OF FORESTRY
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