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Srm/mrm assay for the tubulin beta-3 chain (TUBB3) protein

A protein, protein technology, applied in the determination/inspection of microorganisms, preparation of test samples, labels used in chemical analysis, etc., can solve the problem of not being considered cancer cells, etc.

Inactive Publication Date: 2019-06-07
EXPRESSION PATHOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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However, these agents also kill growing normal cells and are therefore not considered a "targeted" way to kill cancer cells

Method used

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  • Srm/mrm assay for the tubulin beta-3 chain (TUBB3) protein
  • Srm/mrm assay for the tubulin beta-3 chain (TUBB3) protein
  • Srm/mrm assay for the tubulin beta-3 chain (TUBB3) protein

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Embodiment Construction

[0026] The assays described herein measure relative or absolute levels of specific unmodified peptides from the TUBB3 protein. Relative quantitative levels of TUBB3 protein can be determined by SRM / MRM methods, e.g., by comparing SRM / MRM signature peaks (e.g., signature peak areas or integrated fragmention intensities) of individual TUBB3 peptides in different samples . Alternatively, multiple SRM / MRM peak regions of multiple TUBB3 signature peptides, each with its own specific SRM / MRM signature, can be compared to determine the relative TUBB3 protein content in a biological sample , and compare it with the TUBB3 protein content in one or more additional or different biological samples. In this manner, the amount of one or more specific peptides from a TUBB3 protein is determined relative to the same one or more TUBB3 peptides across two or more biological samples under the same experimental conditions, and determined by The amount of this TUBB3 protein. Furthermore, compar...

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Abstract

The current disclosure provides for a specific peptide, and derived ionization characteristics of a peptide from the tubulin beta-3 chain protein (TUBB3) that is particularly advantageous for quantifying the TUBB3 protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry. A protein sample is prepared from a biological sample using the Liquid Tissue reagents and protocol and the TUBB3 protein is quantitated by SRM / MRM mass spectrometry analysis of the sample, where the specific peptide is quantitated. Methods oftreatment are provided in which the measured level of TUBB3 in a patient tumor sample is compared with a reference level and the patient is treated with a taxane-based treatment regimen when the measured TUBB3 level is lower than the reference level. A suitable reference level is, for example, about 700 amol / [mu]g tissue.

Description

[0001] This application claims priority to U.S. Provisional Application Nos. 62 / 384,202, filed September 7, 2016, and 62 / 402,984, filed September 30, 2016, both titled for Tubulin beta-3 SRM / MRM Assay for Chain (TUBB3) Protein, the entire contents of which are hereby incorporated by reference in their entirety. Background technique [0002] Cancer is treated with therapeutic agents that work in a variety of ways to kill growing and dividing cells. A common set of chemotherapeutic agents has been used for decades, either alone or in combination, and has become traditional and routine cancer treatment in clinical oncology practice. These drugs usually work by killing all rapidly dividing cells, one of the main properties of most cancer cells. However, these agents also kill growing normal cells and are therefore not considered a "targeted" way to kill cancer cells. In recent years, a large number of cancer therapies have been developed that specifically target only cancer cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6848G01N2800/52G01N33/6851G01N2458/15G01N2560/00G01N2800/56C12Q1/37G01N1/30G01N30/72G01N2001/305
Inventor 托德·亨布罗法比奥拉·塞奇沙力特·施瓦兹
Owner EXPRESSION PATHOLOGY
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