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Preparation method of mixed dye-labeled fluorescent microsphere

A technology of mixing dyes and marking fluorescence, which is applied to chemical instruments and methods, luminescent materials, etc., can solve the problems of shortage, high price, and short supply, and achieve the effect of simple production method, high safety factor, and uniform size and structure

Inactive Publication Date: 2019-06-04
湖北新纵科病毒疾病工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, judging from the current development at home and abroad, it is still far from enough to achieve a wider and more efficient application of fluorescent microspheres.
Especially in China, the currently used fluorescent microspheres basically depend on importing some large foreign manufacturers, which is not only expensive, but also often in short supply.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method for mixing dye-labeled fluorescent microspheres, comprising the following steps:

[0030] S1, preparation of carboxylated porous polystyrene microspheres, standby:

[0031] S11. Add 1 part by weight of blank PS microspheres to 200 parts by weight of 0.25Wt.% SDS aqueous solution, place it under the condition of 100W and ultrasonically disperse it for 20 minutes, and obtain A dispersion liquid, which is set aside;

[0032] S12. Add 1 part by weight of cyclohexane into 200 parts by weight of 0.25Wt.% SDS aqueous solution, place it under the condition of 100W and ultrasonically disperse it for 20 minutes to obtain the B dispersion, which is set aside;

[0033] S13. Add the B dispersion liquid obtained in the step S12 to the A dispersion liquid obtained in the step S11 dropwise, keep stirring during the addition process, then place it at 10°C for swelling for 4 hours, and add 1 wt. The BPO of part, the styrene of 100 parts by weight, the ethylene glyco...

Embodiment 2

[0042] A preparation method for mixing dye-labeled fluorescent microspheres, comprising the following steps:

[0043] S1, preparation of carboxylated porous polystyrene microspheres, standby:

[0044] S11. Add 3 parts by weight of blank PS microspheres to 600 parts by weight of 0.25Wt.% SDS aqueous solution, place it under the condition of 300W and ultrasonically disperse for 30 minutes, and obtain A dispersion liquid, which is set aside;

[0045] S12. Add 1-5 parts by weight of cyclohexane into 600 parts by weight of 0.25Wt.% SDS aqueous solution, place it under the condition of 300W and ultrasonically disperse for 30 minutes, and obtain the B dispersion, which is set aside;

[0046] S13. Add the B dispersion liquid obtained in the step S12 to the A dispersion liquid obtained in the step S11 dropwise, keep stirring during the addition process, then place it at 30°C for swelling for 8 hours, and add 3 wt. The BPO of part, the styrene of 300 parts by weight, the ethylene glycol ...

Embodiment 3

[0055] A preparation method for mixing dye-labeled fluorescent microspheres, comprising the following steps:

[0056] S1, preparation of carboxylated porous polystyrene microspheres, standby:

[0057] S11. Add 5 parts by weight of blank PS microspheres to 1000 parts by weight of 0.25Wt.% SDS aqueous solution, place it under the condition of 500W and ultrasonically disperse for 40 minutes, and obtain A dispersion liquid, and set aside;

[0058] S12. Add 1-5 parts by weight of cyclohexane into 1000 parts by weight of a 0.25Wt.% SDS aqueous solution, place it under the condition of 500W and ultrasonically disperse for 40 minutes, and obtain the B dispersion, which is set aside;

[0059] S13. Add the B dispersion liquid obtained in the step S12 to the A dispersion liquid obtained in the step S11 dropwise, keep stirring during the addition process, then place it at 50°C for swelling for 12 hours, and add 5 wt. The BPO of part, the styrene of 500 parts by weight, the ethylene glyco...

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PUM

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Abstract

The invention discloses a preparation method of a mixed dye-labeled fluorescent microsphere, and the preparation method comprises the following steps: S1, preparing carboxylated porous polystyrene microspheres for later use; S2, selecting at least two different fat-soluble fluorescent dyes to prepare a mixed dye for later use; and S3, and embedding the mixed dye obtained in the step S2 into the fluorescent microspheres so as to obtain the fluorescent microspheres. The invention provides a preparation method of the fluorescent microsphere by simultaneously using at least two different fat-soluble fluorescent dye-marked polystyrene microspheres.

Description

technical field [0001] The invention relates to the field of nano biological materials. More specifically, the present invention relates to a method for preparing fluorescent microspheres labeled with mixed dyes. Background technique [0002] With the continuous development of materials science, interdisciplinary research between various disciplines. How to endow materials with more detectable and identifiable information is also a requirement for more and more informatized and digital information materials to be continuously researched and applied. [0003] Fluorescent microspheres are a kind of functional microspheres with fluorescent properties, which often have the characteristics of stable morphological structure, uniform size, good dispersion, and stable and efficient luminous efficiency. It is precisely because of these excellent characteristics that fluorescent microspheres have attracted more and more attention from scientific researchers, and have been applied in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02
Inventor 王华林张涛张科登韩迪
Owner 湖北新纵科病毒疾病工程技术有限公司
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