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High performance liquid chromatographic analysis method of benzoic acid and sorbic acid in toothpaste

A technology of high-performance liquid chromatography and analysis method, which is applied in the field of high-performance liquid chromatography analysis of benzoic acid and sorbic acid in toothpaste, can solve the problem that there is no relevant analysis method for benzoic acid and sorbic acid, and achieve reliable and accurate quantification The effect of accurate analysis and detection results

Inactive Publication Date: 2019-05-31
JIANGSU SUPERVISION & INSPECTION INST FOR PROD QUALITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for quicker analysis of both acids (benzeneacetic acid) and gases called sorbitol). By combining these two substances together with other chemicals like salicylic acid or citric acid into one solution that works effectively at different levels within dental healthcare facilities' offices, this process becomes more efficient than current techniques such as gas absorption spectrometry.

Problems solved by technology

This patents describes different ways how people use or handle certain materials like dental flosses during cleaning teeth. These products can contain various types of bacteria such as saliva from cariogenesis (which causes bad breath) along their way through them. There may be concerns about these harmful microorganisms if they enter our bodies' digestive tract due to improper handling techniques. Therefore it becomes important to have an accurate understanding on this issue by analyzing what type of material we consume together before making any changes over time.

Method used

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  • High performance liquid chromatographic analysis method of benzoic acid and sorbic acid in toothpaste
  • High performance liquid chromatographic analysis method of benzoic acid and sorbic acid in toothpaste
  • High performance liquid chromatographic analysis method of benzoic acid and sorbic acid in toothpaste

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Embodiment 1

[0065] Standard working solution preparation:

[0066] The above-mentioned 100mg / L mixed standard stock solution was diluted step by step to configure 50, 20, 10, 5mg / L standard working solutions. Filter through a 0.45 μm membrane filter for later use.

[0067] Sample preparation:

[0068]After the toothpaste sample is squeezed out about 20mm, accurately weigh 1.0000g (accurate to 0.0002g) of a uniform sample into a colorimetric tube (25mL), add 10mL of pure water, oscillate and ultrasonically for 30 minutes, adjust the pH value to 7 with ammonia water, and oscillate for 15 After 10 minutes, add pure water to make up to 25mL, shake well and let it stand still. After standing still, transfer the supernatant to a centrifuge tube, centrifuge at 4000r / min for 10 minutes, take the supernatant and pass it through a 0.45um microporous filter membrane before injecting a sample for detection .

[0069] Blank sample preparation:

[0070] Except that no sample was added, all the trea...

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Abstract

The invention discloses a high performance liquid chromatographic analysis method of benzoic acid and sorbic acid in toothpaste and belongs to the technical field of analysis. High performance liquidchromatography is adopted to respectively detect a blank sample, a sample solution and a standard curve solution, and the blank sample, a standard curve, a sample, a sample and a standard curve are sequentially detected (notes: the number of feeding the sample between the two standard curves should not exceed 15, and a quality control sample or a standard curve needs to be added therebetween if the number exceeds 15). The method can quantitatively analyze content of benzoic acid and sorbic acid in a toothpaste sample quickly and accurately and can be used by market regulatory authorities for monitoring quality of commercially available toothpaste products. The method has the advantages of accurate detection result and reliability.

Description

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Claims

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Application Information

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Owner JIANGSU SUPERVISION & INSPECTION INST FOR PROD QUALITY
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