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Preparation method and application of Providencia stuartii immobilized bacterium

A technology of Providencia stutzeri and Providencia, applied in the field of biotechnology degradation of pesticide residues

Inactive Publication Date: 2019-05-24
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no research work has been carried out on the biodegradation of sclerotin

Method used

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  • Preparation method and application of Providencia stuartii immobilized bacterium
  • Preparation method and application of Providencia stuartii immobilized bacterium

Examples

Experimental program
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Effect test

Embodiment 1

[0018] 1. Screening of immobilized carriers:

[0019] Preliminary experiments have shown that in 1%, 2%, 3%, 4%, 5% sodium alginate (SA) to fix and suspend free cells, 4% SA shows a better effect. Further, the experiment determined the degradation effect of free cells, bamboo charcoal (BC)+free cells, 4%SA+free cells, 4%SA+bamboo charcoal (BC)+free cells on 50mg / L of sclerotia, and the results are shown in figure 1 .

[0020] 2. Preparation of immobilized bacteria:

[0021] 1) Bacterial suspension preparation: Add 2 mL of sterile phosphate buffer under aseptic conditions and shake in parallel in the culture dish cultured for 48 hours, and gently scrape off the colonies on the surface of the medium with an inoculation loop to form a crude bacterial suspension. liquid, and transferred it to a liquid fermentation medium (beef extract 8g / L, yeast extract 14g / L, NaCl 5g / L) and cultured for 48h at pH 7-8, 30-35°C, 150-200rpm. Immediately centrifuge the fermented broth taken out a...

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Abstract

The invention discloses a preparation method and an application of a Providencia stuartii immobilized bacterium. The immobilized bacterium takes Providencia stuartii as a strain and takes sodium algaacid and bamboo charcoal as immobilized materials, and the providencia stuartii is effectively embedded to prepare the immobilized bacterium. The collection number of the Providencia stuartii strain is CCTCC M2017738. The immobilized bacterium can be applied to high-effective degradation of dimethachlon, vinclozolin and iprodione in soil and water. The immobilized bacterium has the advantages of good persistence, environmental friendliness, remarkable effect, low cost, easiness in popularization and the like.

Description

technical field [0001] The invention relates to the field of biotechnological degradation of pesticide residues, in particular to a preparation method and application of an immobilized bacterium of Providencia stutzeri. Background technique [0002] Pesticides play an important role in the integrated management of agricultural pests, but pesticide residues and their metabolites have become serious pollutants, posing threats to human health and ecosystems. Dimetachlone, also known as Dimetachlone, has molecular formula C 10 h 7 Cl 2 NO 2 , its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl)succinimide. In my country, it is widely used in the prevention and control of fungal diseases such as sclerotinia, sheath blight, and red spot on rapeseed, Chinese cabbage, laver moss, lettuce, leek, cucumber, tomato, pepper, potato, tobacco and other crops. Despite its promising bactericidal activity, sclerotinia is currently banned in the US and E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C02F3/34B09C1/10C12R1/01C02F101/34C02F101/38
Inventor 吴小毛张承李荣玉龙友华李姣红王秋萍黄文源代园凤
Owner GUIZHOU UNIV
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