Method and system for identifying Chinese population epithelial cell body fluid spot tissue source from gene level

Abstract

The invention provides a method and system for identifying a Chinese population epithelial cell body fluid spot tissue source from the gene level. The method comprises the steps of utilizing a first PCR amplification primer set for performing first amplification on body fluid spot DNA, and obtaining a first amplification PCR product, wherein the first PCR amplification primer set is composed of nine pairs of amplification primers corresponding to nine genes of CYP2B7P1, MUC4, L.cri, HTN3, STATH, MUC7, LCE2B, DCD and CCL27 one to one; according to the gene expression condition in the first amplification PCR product, whether or not at least one of vaginal secretion, saliva and skin is contained in body fluid spots is judged. According to the scheme, the Chinese population body fluid tissue source can be effectively determined from the gene level, the step for identifying the body fluid or spot tissue source thereof at a criminal investigation site is greatly simplified, and the accuratescientific basis can be supplied to case character clearing, conviction and sentencing.

Description

technical field [0001] The present invention relates to a method and a system for the source of human body fluid tissue in a population, in particular to a method and a system for identifying the source of epithelial cell body fluid spot tissue in a Chinese population at the genetic level. Background technique [0002] Body fluid stains are common biological evidence at crime scenes, which can be analyzed using deoxyribonucleic acid (DNA) identification technology to identify or exclude suspects. In recent years, with the deepening of the reform of the litigation system, only obtaining DNA typing from biological evidence to determine the individual source is increasingly unable to meet the integrity requirements of the evidence, and this single result has also caused some controversy. The identification of traces is precisely an important part of the controversy. Traditional body fluid spot detection methods, such as salivary amylase test, mainly judge the existence of the ...

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Problems solved by technology

[0005] The above-mentioned saliva, vaginal secretions, and skin contact spots are the focus of this research. These three body fluid spots have certain commonalities: they all contain cells of epithelial origin, and the cell functions are similar, and the expressed proteins also have certain similarities. In addition, related studies have also proved that some specific mRNA markers can be detected in the above-mentioned 2-3 kinds of body fluid spots at the same time, which may cause false positive results and affect the interpretation of identification results

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