Genital tract flora nucleic acid preservation solution formula and preparation and application method

Abstract

The invention relates to a genital tract flora nucleic acid preservation solution formula. A genital tract flora nucleic acid preservation solution comprises a Tris-HCl buffer solution with the concentration of 0.01-1 M, ethylenediaminetetraacetic acid with the concentration of 0.01-1 M, sodium chloride with the concentration of 0.01-0.5 M, sodium citrate with the concentration of 0.01-0.5 M and 10-30% anhydrous ethanol. The pH value of the prepared preservation solution is 8.0-10.0. The preservation solution is prepared through the method and used for collecting a genital tract sample, has the advantages of protecting microorganisms and nucleic acids thereof in the genital tract from being degraded, protecting the microbial diversity, facilitating collection and transportation of the genital tract sample and facilitating downstream gene detection, is beneficial to genital tract flora diversity research, and is applied to gene detection by taking genital tract floras as target points.

Description

technical field [0001] The invention relates to the technical field of microbes, and more specifically, to a formula, preparation and use method of a genital tract flora nucleic acid preservation solution. Background technique [0002] Bacteria inhabit almost every part of the human body, and the number is in the trillions, up to 1014, which is about 10 times the total number of human cells. It is roughly estimated that there are nearly a thousand species of bacteria in the human gastrointestinal tract. Compared with the intestinal flora, the types of vaginal flora are simpler. There can be about 40 genera and more than 100 species of bacteria on the side wall mucosa around the vagina of women of childbearing age, weighing up to 20 grams. Including Lactobacillus, Atopobium vaginalis, Macrococcus, Corynebacterium, Escherichia coli, Veillonella and Gardnerella, etc. These vaginal bacteria can be roughly divided into two categories: "beneficial bacteria" and "conditional path...

AI Technical Summary

Problems solved by technology

However, in the process of collection and transportation, the microorganisms and their nucleic acid composition in the reproductive tract samples are affected by the following factors: First, most of the reproductive tracts are anaerobic bacteria, and due to changes in the oxygen content in the environment, these bacteria may rapidly Stop growing and die, causing its cell wall to rupture and nucleic acid to degrade; second, changes in temperature will also cause changes in the growth rate of different bacteria, which will change the composition of the reproductive tract flora, and some temperature-sensitive microbial species will die and decompose, and nucleic acid Degradation disappears

The conventional storage method, that is, ultra-low temperature (-80°C) storage is not suitable for routine home sampling and transportation, causing some users to give up testing or the test results are inconsistent with the actual situation

Images