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Method for enriching listeria monocytogenes in meat and meat products

A technology for Listeria monocytogenes and Listeria, applied in the field of detection, can solve the problems of endangering consumer safety, cumbersome operations and steps, false negative results, etc., to ensure effectiveness, ensure safety, and increase detection rate effect

Pending Publication Date: 2019-04-26
HENAN BUSINESS SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation and steps are very cumbersome and time-consuming, and only the surviving L. monocytogenes in the sample can be detected. The detection rate is lower than that of the actual L. monocytogenes in the sample, and it is very easy to produce false negative results, which endangers the majority of consumers. Safety

Method used

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  • Method for enriching listeria monocytogenes in meat and meat products
  • Method for enriching listeria monocytogenes in meat and meat products
  • Method for enriching listeria monocytogenes in meat and meat products

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Embodiment 1

[0024] A method for enriching Listeria monocytogenes in meat and meat products, comprising the following steps:

[0025] 1) Aseptically take 25g of meat and meat product samples, add 225mL LB 3 +Hanks (1:1) buffer solution, use a homogenizer to pulverize and homogenize for 5 minutes, and the homogenized sample solution is ready for use; take the sample solution and let it stand at a low temperature for 60 minutes at 2°C to make the fat and protein components in the sample Coagulate under conditions to remove most of the fat and protein, and homogenize the remaining sample for later use; centrifuge at 4°C and 10,000rmp / min for 10min under sterile low temperature conditions to further remove remaining fat, protein and other macromolecular substances, and collect the supernatant after centrifugation for later use;

[0026] 2) Take the supernatant from 1), and filter it with a Φ50mm 0.8μm polyether PES microporous membrane to enrich the microorganisms on the microporous membrane; ...

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Abstract

The invention discloses a method for enriching listeria monocytogenes in meat and meat products. The method comprises the following steps: firstly, homogenizing meat and meat product samples, combining low temperature static pretreatment with low-temperature high-speed centrifugation to remove large molecular component disruptors such as rich fat and protein in the samples; enriching target bacteria in the samples on a microfiltration membrane by adopting a microfiltration membrane filtration method. The detection limit of the listeria monocytogenes in the meat and meat products is improved, so that the detection problem of low level of the listeria monocytogenes in the meat and meat products is solved. Target bacteria in a filter membrane and filter membrane aperture are eluted by using eluent favorable for growth and protection of the listeria monocytogenes; by using specific combination of listeria monocytogenes immunomagnetic beads with listeria monocytogenes in bacterial suspension, the aim of enriching the listeria monocytogenes in the meat and meat products is achieved and the detection rate of the listeria monocytogenes is increased.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for enriching Listeria monocytogenes in meat and meat products. Background technique [0002] Food safety is related to the national economy and people's livelihood. Foodborne diseases and food contamination are important factors that endanger public health and are the most concerned public safety issues in the world today. From 2001 to 2010, foodborne diseases caused by microbial contamination in my country accounted for 56.39% of the total. The analysis of epidemiological monitoring data also shows that the incidence of bacterial foodborne diseases in my country can reach 115.017 million per year, the fatality rate is 0.0074%, and the underreporting rate is estimated to reach 99.992%. Listeria monocytogenes (Listeria monocytogenes) referred to as Listeria monocytogenes, intracellular parasitic Gram-positive bacilli, is a zoonotic pathogen, can still grow...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02G01N33/569C12R1/01
CPCG01N33/56911C12N1/02C12N1/20G01N2333/195
Inventor 朱海华王法云王永周莉谭静平洋王慧王晓瑞李鹏冲张亚勋高海东
Owner HENAN BUSINESS SCI RES INST
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