Method for enriching listeria monocytogenes in meat and meat products
A technology for Listeria monocytogenes and Listeria, applied in the field of detection, can solve the problems of endangering consumer safety, cumbersome operations and steps, false negative results, etc., to ensure effectiveness, ensure safety, and increase detection rate effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] A method for enriching Listeria monocytogenes in meat and meat products, comprising the following steps:
[0025] 1) Aseptically take 25g of meat and meat product samples, add 225mL LB 3 +Hanks (1:1) buffer solution, use a homogenizer to pulverize and homogenize for 5 minutes, and the homogenized sample solution is ready for use; take the sample solution and let it stand at a low temperature for 60 minutes at 2°C to make the fat and protein components in the sample Coagulate under conditions to remove most of the fat and protein, and homogenize the remaining sample for later use; centrifuge at 4°C and 10,000rmp / min for 10min under sterile low temperature conditions to further remove remaining fat, protein and other macromolecular substances, and collect the supernatant after centrifugation for later use;
[0026] 2) Take the supernatant from 1), and filter it with a Φ50mm 0.8μm polyether PES microporous membrane to enrich the microorganisms on the microporous membrane; ...
PUM
Property | Measurement | Unit |
---|---|---|
Aperture | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com