A kind of titanium carbide three-dimensional composite material and its preparation method and its application in the construction of thrombin aptamer sensor

An aptamer sensor and composite material technology, applied in the analysis of materials, material analysis through electromagnetic means, instruments, etc., can solve the problems of cumbersome and complicated processes, achieve accelerated electron transfer rate, good electrochemical performance, and improve charge separation efficiency Effect

Active Publication Date: 2020-06-26
QINGDAO UNIV
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Problems solved by technology

The process of preparing materials is cumbersome and complicated

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  • A kind of titanium carbide three-dimensional composite material and its preparation method and its application in the construction of thrombin aptamer sensor
  • A kind of titanium carbide three-dimensional composite material and its preparation method and its application in the construction of thrombin aptamer sensor
  • A kind of titanium carbide three-dimensional composite material and its preparation method and its application in the construction of thrombin aptamer sensor

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preparation example Construction

[0034] In a first exemplary embodiment of the present disclosure, a M NPs / TiO 2 / Ti 3 C 2 T X A method for preparing a composite material, the method comprising:

[0035] layered Ti 3 C 2 T X , noble metal salt and water are mixed evenly, then hydrothermally reacted at 160-200°C for 12-16 hours, cooled and left to stand for 12-15 hours to prepare M NPs / TiO 2 / Ti 3 C 2 T X composite material.

[0036] The present disclosure adopts a one-pot hydrothermal method to directly reduce noble metal nanoparticles (M NPs) to the composite material TiO 2 / Ti 3 C 2 T X At the same time, after the end of the hydrothermal reaction, the TiO 2 Growth of nanorods to obtain TiO 2 It is a nanorod structure and its size is relatively large. The inventor unexpectedly found that this structure is more conducive to stretching Ti 3 C 2 T X The layered structure is conducive to improving the proton transmission rate, which is more conducive to the detection of thrombin.

[0037] Afte...

Embodiment 1

[0069] MXenes-Ti 3 C 2 T X Synthesis

[0070] 3g Ti 3 AlC 2 Slowly add 30 mL of 9M HF solution at 5°C to obtain a mixed solution. The mixed solution was stirred at 5 °C for 20 min and then at 45 °C for 24 h to obtain Ti 3 C 2 T X suspension of flakes. It was then centrifuged until the pH of the suspension reached 6.5, layered Ti was obtained by sonication for 11 h 3 C 2 T X The colloidal solution was then centrifuged at 3500 rpm for 35 minutes to remove the precipitate.

[0071] TiO 2 / Ti 3 C 2 T X Synthesis of Hydrogels

[0072] 20mL (4mg / mL) Ti 3 C 2 T X The solution was dispersed in 5 mL of water and sonicated for 1.2 h to obtain a homogeneous suspension. Then, the suspension was transferred into a Teflon sealed autoclave and heated at 180 °C for 14 h. After cooling to room temperature, let it stand for 14 hours to form TiO 2 Growth of nanorods. Synthesized three-dimensional composite nanomaterial TiO 2 / Ti 3 C 2 T X Wash with water, then store in...

Embodiment 2

[0090] MXenes-Ti 3 C 2 T X Synthesis

[0091] 3g Ti 3 AlC 2 Slowly add 30 mL of 9M HF solution at 5°C to obtain a mixed solution. The mixed solution was stirred at 5 °C for 20 min and then at 45 °C for 24 h to obtain Ti 3 C 2 T X suspension of flakes. It was then centrifuged until the pH of the suspension reached 6.5, layered Ti was obtained by sonication for 11 h 3 C 2 T X The colloidal solution was then centrifuged at 3500 rpm for 35 minutes to remove the precipitate.

[0092] TiO 2 / Ti 3 C 2 T X Synthesis of Hydrogels

[0093] 20mL (4mg / mL) of Ti3 C 2 T X The solution was dispersed in 5 mL of water and sonicated for 1.2 h to obtain a homogeneous suspension. Then, the suspension was transferred into a Teflon sealed autoclave and heated at 180 °C for 14 h. After cooling to room temperature, let it stand for 14 hours to form TiO 2 Growth of nanorods. Synthesized three-dimensional composite nanomaterial TiO 2 / Ti 3 C 2 T X Wash with water, then store i...

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Abstract

A titanium carbide three-dimensional composite material, a preparation method therefor, and application thereof in constructing a thrombin aptasensor. A three-dimensional composite material substrate TiO2 / Ti3C2TX is formed by self-growing TiO2 nanorods on the surface of Ti3C2TX with a one-step hydrothermal method, and a three-dimensional composite material M NPs / TiO2 / Ti3C2TX is further obtained by reducing noble metal nanoparticles M NPs on the surface of a three-dimensional material. The composite material has the highest current intensity because a variety of enhancements are achieved in one step. The three-dimensional structure of the composite material can provide a particularly large accessible surface area, which facilitates anchoring of M NPs. The introduction of a transition metal carbonitride MXenes and the metal nanoparticles can improve the efficiency of charge separation and accelerate electron transfer. A sensitive unlabeled aptamer is successfully established by means of the combination of M NPs and aptamer chains, and is used for determining enzyme protein. The provided aptasensor has good electrochemical performance, a wide linear range, and a low detection limit; this indicates that M NPs / TiO2 / Ti3C2TX is promising to be used as an electrode material in an electrochemical biosensor.

Description

technical field [0001] The disclosure specifically relates to a titanium carbide three-dimensional composite material, a preparation method thereof, and an application in constructing a thrombin aptamer sensor. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Thrombin converts fibrinogen to fibrin. After local application, the blood acting on the lesion surface rapidly forms a stable clot, which is used to control capillary, venous bleeding, or as an adhesive and fixative for skin and tissue grafts, and is involved in various diseases such as thromboembolic Diseases, leukemia and inflammatory responses. To adapt to global health concerns, especially in developing countries, it is highly desirable to develop simple, low-cost and effective methods for the quantitative detection of thrombin. Some conventional methods for protein analysis, such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 张菲菲邹慧燕王宗花杨潇
Owner QINGDAO UNIV
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