A kind of screening method of Sihuang anti-acne compound formula
A screening method and compound technology, which is applied in the fields of medical formula, medical preparations containing active ingredients, chemical property prediction, etc., can solve problems such as the lack of clear understanding of the anti-acne efficacy of traditional Chinese medicine compound, and achieve the effect of good technical support
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[0021] Reagents used: Coptis chinensis, Treats, Scutellaria baicalensis, Rhubarb, purchased from Hefei People's Yuan Pharmacy; p-dimethylaminobenzaldehyde, reduced coenzyme II (NADPH, 95%), testosterone (98%), Aladdin reagent (Shanghai) Co., Ltd.; Tris (Tris, 99.9% biotech grade), Coomassie brilliant blue G-250 (AR, no protease), 10mg / mL bovine serum albumin standard solution (analytical standard), Shanghai McLean Biochemical Technology Co., Ltd.; Beef Extract, Beijing Auboxing Biotechnology Co., Ltd.; Sodium Chloride, Peptone, Sinopharm Chemical Reagent Co., Ltd.; Aplite (5mg / tablet, active ingredient content), Jiangsu Lianhuan Pharmaceutical Co., Ltd. Company; 5α-reductase extract, laboratory extraction, the concentration is 0.29mg / mL.
[0022] A screening method for Sihuang acne-removing compound, comprising the following steps:
[0023] (1) Preparation of Chinese herbal water extract of Sihuang: Four Chinese herbal medicines, including Coptis chinensis, Cortex Phellodendr...
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