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Application of hsa-mir-145-5p in the treatment of breast cancer

A breast cancer and sensitivity technology, applied in the biological field, can solve problems such as ambiguity, and achieve the effects of weakening activity, reducing mortality, and reducing drug resistance

Active Publication Date: 2021-04-20
TSINGHUA BERKELEY SHENZHEN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of how miRNA regulates CaN to promote drug resistance in breast cancer is still unclear.

Method used

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  • Application of hsa-mir-145-5p in the treatment of breast cancer
  • Application of hsa-mir-145-5p in the treatment of breast cancer
  • Application of hsa-mir-145-5p in the treatment of breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1 Materials and methods

[0033] 1.1 Main cell line: MCF7.

[0034] 1.2 qPCR experiment

[0035] 1.2.1 Extraction of total cellular RNA

[0036] Cells were collected, added Trizol, mixed by pipetting, and allowed to stand at room temperature for 5 minutes. Centrifuge at 10,000×g for 10 minutes at 4°C, take the supernatant, add 0.2ml of chloroform, shake vigorously for 30s, and let stand for 3 minutes. Centrifuge at 4°C, 12000g×15min, pipette the supernatant into a new DEPC-treated 1.5ml EP tube, add 0.5ml isopropanol, and mix gently. Centrifuge at 4°C, 12000g×10min, discard the supernatant, add 1ml of 75% ethanol (ice-cold), shake, and fully wash the precipitate. Centrifuge at 4°C, 12000g×5min. Discard the supernatant, centrifuge briefly, carefully aspirate and discard the supernatant. Add an appropriate amount (20 μl) of DEPC (Rnase Free) water to dissolve the RNA.

[0037] 1.2.2 qPCR experiment

[0038] (1) According to the predicted results, synthesize the co...

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Abstract

The invention discloses the application of hsa-miR-145-5p in the treatment of breast cancer. The invention finds that increasing the content of hsa-miR-145-5p in tumor cells significantly reduces the drug resistance of cancer cells and weakens the tumor cells activity for the treatment of breast cancer. Combining hsa-miR-145-5p with calcineurin inhibitors may be a means to improve chemotherapy drugs and reduce breast cancer mortality, and greatly improve the effect of anti-tumor drugs on tumor cells.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to tumor molecular markers, in particular to the application of hsa-miR-145-5p in treating breast cancer and improving the sensitivity of breast cancer to therapeutic drugs. Background technique [0002] The latest global statistical analysis of tumors in 2018 covers 185 countries and regions and 36 types of cancer. It mainly analyzes the incidence and mortality of each cancer in 2018, as well as the incidence of tumors in various regions. Statistics show that so far, there are about 18.19 million new cancer cases and 9.6 million cancer deaths worldwide. Asia accounts for about 50% of new cancer cases and 70% of cancer deaths. The final analysis results show that breast cancer is the number one "killer" of women (globally, there will be about 2.1 million newly diagnosed female breast cancer cases in 2018), and among the diagnosed female cancer cases worldwide, breast cancer Accounting for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7105A61P35/00
CPCA61K31/7105A61P35/00
Inventor 黄来强孙春辉代小勇
Owner TSINGHUA BERKELEY SHENZHEN INST
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