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Hematoxylin staining solution and preparation method

A technology of hematoxylin and dyeing solution, which is applied in the preparation, sampling, measuring device and other directions of test samples, can solve the problems of stained sections, difficult to clean, difficult to grasp the degree of oxidation, etc., and achieves bright colors and strong contrast, clear tissue structure, Ideal dyeing effect

Inactive Publication Date: 2019-03-19
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Almost all routine sections, frozen sections, smears and counterstaining after immunohistochemistry use this staining solution. It has many advantages, but there are also unsatisfactory places, such as short use period, and the amount of dyed slides is about one It has to be replaced once a month; there are many oxide crystals, and it must be filtered every time it is used, otherwise a luminous crystal will precipitate on the slice. It is speculated that this substance may be the product of mercury oxide in the oxidation process
In addition, the preparation process is more complicated, and mercury oxide has certain toxicity
There are following four problems in the preparation: one is that the fire source is required to boil, the other is that the oxidant used is mercury oxide, which is easy to cause environmental pollution and have adverse effects on human health, the third is that the preparation process is more complicated and difficult to grasp the degree of oxidation, and the fourth is that A large amount of hematoxylin will easily produce a large number of oxides floating on the surface of the dye solution, staining the slices and making it difficult to wash

Method used

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  • Hematoxylin staining solution and preparation method
  • Hematoxylin staining solution and preparation method

Examples

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Embodiment 1

[0038] Solution A: Mix and dissolve an appropriate amount of hematoxylin 2.5g and pure ethanol 200ml, take an appropriate amount of distilled water and 8.5g of alum in a 1000ml beaker, stir properly, and then add potassium iodate 0.29g and citric acid 2.2ml in a certain order . Sodium nitrite 1.5g. Mix well, and finally, make up to 500ml with distilled water. Stir well. After cooling and filtering, 2.2ml of citric acid was added.

[0039] Solution B (stable dyeing aid): Take 72ml of glycerin, 0.94g of polyoxyethylene type nonionic surfactant, 20ml of ethanol, and 2ml of 1% potassium sorbate solution, mix them evenly and dissolve completely, and place them.

[0040] After the solution A was prepared and left for 48 hours, the solution B was added. At this time, the solution was dark purple, which was a kind of hematoxylin staining solution prepared as described above. Storage conditions: This reagent should be stored away from light in an environment with a relative humidit...

Embodiment 2

[0043] Solution A: Mix and dissolve an appropriate amount of hematoxylin 3.3g and pure ethanol 200ml, take an appropriate amount of distilled water and 15.5g of alum in a 1000ml beaker, stir properly, and then add potassium iodate 0.35g, sodium nitrite 1.9 g. Mix well, and finally, make up to 500ml with distilled water. Stir well. After cooling and filtering, 2.7ml of citric acid was added.

[0044] Liquid B (stable dyeing aid): get 80ml of glycerin, 1.9g of polyoxyethylene type nonionic surfactant, 20ml of ethanol, 2.5ml of 1% potassium sorbate solution, mix evenly and dissolve completely, and place it.

[0045] After the solution A was prepared and left for 48 hours, the solution B was added. At this time, the solution was dark purple, which was a kind of hematoxylin staining solution prepared as described above. Storage conditions: This reagent should be stored away from light in an environment with a relative humidity of not more than 80%, no corrosive gas and good ventil...

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Abstract

The invention relates to the field of biomedical reagents and in particular relates to a hematoxylin staining solution and a preparation method. The formula usage amount is reasonable, and the staining effect is ideal. The tissue slice stained by the hematoxylin staining solution provided by the invention is excellent in cell nucleus staining and clear in structure, and the tissue slice stained byeosin is bright in color, strong in contrast and easy to observe, and the staining effect is ideal. The staining method of the hematoxylin staining solution provided by the invention is simple and easy to operate, heating is not needed in the preparation process, the staining process is safe, and the tissue slices are not contaminated.

Description

technical field [0001] The invention relates to the field of biomedical reagents, in particular to a hematoxylin staining solution for medical biological cells and tissues and an application reagent for the staining method. Background technique [0002] Hematoxylin staining has been used in biological staining for more than 100 years. It is one of the most commonly used staining methods in histochemistry and immunohistochemistry. , immunofluorescence, in situ hybridization, etc. Hematoxylin staining solution is mainly used to display the normal components of various tissues and the general morphological structure of lesions for comprehensive observation. Hematoxylin staining is the most basic staining method necessary for biology, histology, pathology and cytology. It is widely used in pathological diagnosis, teaching and scientific research, and has important value. Such as the commonly used Hat.ris preparation method, its formula is as follows: A liquid: hematoxylin 1g....

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 王洁迟庆刘玉华鲍一凡王亚东
Owner DALIAN UNIV
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