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Compositions and methods of treatment of chronic infectious diseases

A kind of technology of composition and medicine, applied in the field of pharmaceutical composition of chronic disease, the field of pharmaceutical composition of infection

Inactive Publication Date: 2019-03-01
托马斯·朱利叶斯·波洛迪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is not suitable for the identification of intervening bacteria using human MAP because the culture takes several months to grow

Method used

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  • Compositions and methods of treatment of chronic infectious diseases
  • Compositions and methods of treatment of chronic infectious diseases
  • Compositions and methods of treatment of chronic infectious diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Selection of MAP Intervention Bacteria from Cattle

[0050] The following examples describe exemplary methods for selecting therapeutic combinations of intervening bacteria to treat M. avium subsp. paratuberculosis (MAP) in cattle.

[0051] MAP can be grown from a variety of sources to cover a variety of cattle, preferably across many farms and many countries. Blood from these cows can be split into several different test tubes and various concentrations of (exemplary) Dietzella sp. from 10 2 to 10 15 Added to several tubes, but added saline vehicle only to control tubes. After 8 or 20 days of incubation, MAP proliferation within the stained macrophages was examined microscopically to see if a particular Dietzia species selected from a particular cow inhibited MAP. Microscopic screening assays for macrophage proliferation using MAPs save a considerable amount of time required for MAPs grown in culture.

[0052] The next stage is cultivation on slants sui...

Embodiment 2

[0054] Example 2: Selection of human anti-MAP intervention strains

[0055] The following examples describe exemplary methods for selecting therapeutic combinations of intervening bacteria to treat M. avium subsp. paratuberculosis (MAP) in humans.

[0056] Blood was collected from patients with Crohn's disease and cultured for 10-20 days with macrophage strains of MAP in the presence and absence of Dietzella or other candidate inhibitory strains, e.g., selected from Example 1 strains of the method described. Multiple Dietzia strains were tested for each patient, and then the most effective Dietzia inhibitors were combined in a panel of 6-10 Dietzia strains for use as oral treatments. The greater the number of human strains co-cultured with Dietzella, the greater the coverage of probiotic inhibition in Crohn's disease. Rising concentrations of Dietzella during blood incubation will also help determine the titer at which Dietzia will begin to inhibit infection with MAP. Her...

Embodiment 3

[0073] Example 3: Selection of MAP-intervention bacteria from sheep

[0074] The following examples describe exemplary methods for selecting therapeutic combinations of intervening bacteria to treat Mycobacterium avium subtype paratuberculosis (MAP) in sheep (Johne's disease of sheep).

[0075] The same exemplary procedure can be repeated in sheep in a similar manner as described for humans and cattle. However, in cattle and sheep, MAP can actually be grown in the lab, so an intervention to suppress bacteria can be tested on sheep and cattle MAP, and it can be more easily tested on human MAP, which would not be so easy Grow in laboratories using solid media or even liquid media.

[0076] In an alternative embodiment, methods for culturing ovine and bovine MAPs that are also suitable for the growth of M. tuberculosis, on HEYM slants and modified Middlebrook 7H10 agar medium can be used to produce MAP slants. Therefore, these media in screw cap tubes will contain M. paratube...

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Abstract

In alternative embodiments, provided are novel applications of bacteria which originate from the phylum of Actinobacteria and sub-order Corynebacterineae, family Dietziaceae, including genus Dietzia and other genera. Such bacilli can profoundly interfere with bacteria generally belonging to this and other phyla, and can be useful in treating chronic infections. Hence, such organisms can ameliorateor cure clinical infections caused by pathogens from the phylum such as Mycobacteriaceae and Mycobacterium such as M. tuberculosis and Mycobacterium avium subspecies paratuberculosis (MAP).

Description

[0001] Cross-Referenced Related Applications [0002] This application claims priority to patent application USSN 62 / 299,915 filed February 25, 2016, the contents of which are incorporated herein in their entirety. technical field [0003] The present disclosure relates to pharmaceutical compositions for the treatment of chronic conditions in mammals that are frequently exposed to infectious agents. In particular, provided herein are pharmaceutical compositions and methods for treating infections transmitted by acid-fast bacilli and other mycobacterium-like agents in humans and non-human mammals. The present invention provides new applications for bacteria derived from the phylum Actinobacteria and suborder Corynebacterineae, family Dietziaceae, including the genus Dietzia and other genera. This bacillus can deeply interfere with bacteria that normally belong to this and other phyla and can be used to treat chronic infections. Accordingly, the pharmaceutical compositions and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P1/00A61P31/04A61P37/00
CPCA61K9/00A61K45/06A61K9/0095A61K35/74A61P1/00A61P31/04A61P37/00Y02A50/30A61K35/37A61K35/741A61K2035/115
Inventor 托马斯·朱利叶斯·波洛迪
Owner 托马斯·朱利叶斯·波洛迪
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