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Application of specific lectin combination in identifying esophageal cancer based on saliva sugar types

A lectin and esophageal cancer technology, applied in the field of identification of esophageal cancer, can solve the problems that have not yet seen research results

Active Publication Date: 2019-02-15
深圳格道糖生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no research results on salivary sugar chain biomarkers of esophageal cancer

Method used

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  • Application of specific lectin combination in identifying esophageal cancer based on saliva sugar types
  • Application of specific lectin combination in identifying esophageal cancer based on saliva sugar types
  • Application of specific lectin combination in identifying esophageal cancer based on saliva sugar types

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. Experimental part

[0026] 1.1 Reagents and materials

[0027] Epoxy silane reagent (GPTS), Bradford reagent, protease inhibitors, DMSO, Tween-20, and hydroxylamine hydrochloride were all purchased from Sigma Company of the United States, Cy3 and Cy5 fluorescent dyes were constructed from Amerhsam Company of the United States, and Sephadex G-25 column was purchased from the United States GE Healthcare, the 384-well plate was constructed from Genetix, UK, bovine serum albumin (BSA) was purchased from Calbiochem, Germany, the glass substrate was purchased from Gold Seal, the 0.2 μm filter membrane, and the 0.45 μm filter membrane were purchased from Sartorius, Japan. Other commonly used reagents were purchased from domestic analytical grade. Thirty-seven lectins (see Table 1) were purchased from Vector Company and Sigma Company respectively.

[0028] Table 1 Lectin comparison table

[0029]

[0030]

[0031] 1.2 Experimental Instruments

[0032] Electric blas...

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Abstract

The invention provides an application of a specific lectin combination in identifying an esophageal cancer based on saliva sugar types. The specific lectin combination (identified sugar chain) servesas a biomarker for identifying an esophageal cancer based on saliva sugar types and comprises an up-regulation group and a down-regulation group. The up-regulation group comprises Jacalin, ECA, HHL, GSL-II, MAL-II, EEL, RCA120, DSA, NPA, and MAL-I. The down-regulation group comprises SJA, LEL, LCA, STL, ConA, PSA, ACA, PWM, and GNA. When all the sugar chains identified by each lectin of the up-regulation group are up-regulated, and all the sugar chains identified by each lectin of the down-regulation group are down-regulated, the corresponding individual is indicated to have an esophageal cancer. According to the application of a specific lectin combination in identifying an esophageal cancer based on saliva sugar types, the non-injury, high throughput, and rapid identification of an esophageal cancer can be realized.

Description

technical field [0001] The invention relates to a method for identifying esophageal cancer through saliva samples, in particular to the application of determining a specific set of lectins in identifying esophageal cancer based on salivary glycoforms. Background technique [0002] Esophageal cancer (EC) is a malignant tumor originating from the esophageal mucosal epithelium, and is one of the most common malignant tumors in clinic. The incidence of all cancers in the world ranks seventh (572000), and the death rate ranks sixth among all cancers (509000). Among them, 70% of EC patients are men, and the incidence rate of men is 2-3 times that of women, and there are obvious regional differences. EC is highly prevalent in countries in East Asia and South Africa. my country is one of the countries with the highest incidence of esophageal cancer, with more than 220,000 new cases of esophageal cancer and about 200,000 deaths each year. At present, more than 90% of esophageal ca...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57407G01N2800/60
Inventor 李铮舒健张坤于汉杰郭向前杨泽堃
Owner 深圳格道糖生物技术有限公司
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