A kind of dynamic cell culture apparatus and cell culture system

Abstract

The present invention provides a dynamic cell culture device and a cell culture system. The dynamic cell culture device includes a rectangular parallelepiped housing, and the inside of the housing is provided with multi-layer culture chambers distributed from bottom to top and parallel to each other. The two opposite sides of the casing are respectively provided with a liquid inlet chamber and a liquid outlet chamber. The other two opposite sides of the body are respectively provided with a liquid inlet (11) and a liquid outlet (31), and the connection line between the liquid inlet (11) and the liquid outlet (31) is the shell The body diagonal of the body, the liquid inlet (11) communicates with the liquid inlet chamber, and the liquid outlet (31) communicates with the liquid outlet chamber. The dynamic cell culture device provided by the present invention has high convenience in use, is suitable for manual operation, and can reduce the risk of contamination in the cell culture process, and can realize harvesting of a large number of cells in one culture.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a dynamic cell culture device and a cell culture system. Background technique [0002] In the past ten years, due to the rapid development of the biological products industry, the traditional method of obtaining biological products from animal tissues through biochemical technology has been far from meeting the needs of the market. Large-scale culture of animal cells in vitro to express specific proteins, Monoclonal antibody, interferon and virus vaccine products have become the most common technology at present. [0003] Currently commonly used cell culture devices include conventional cell culture dishes, plates, bottle cultures, spinner bottle cultures, large bioreactors, etc. The above cell culture devices have the following defects: [0004] 1) The surface area for cell growth per unit volume is small, the cell growth density is low, and the number of cells harvested i...

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Problems solved by technology

[0004] 1) The surface area for cell growth per unit volume is small, the cell growth density is low, and the number of cells harvested in a single culture is small. To obtain a large number of cells requires a large increase in the number of devices and repeated cultures, and repeated cultures require a lot of materials and labor. and time;

[0005] 2) During the cultivation process, the preparation of culture medium, addition of liquid, and harvesting of cells and cultures are all manual operations step by step. There is a lack of a fully enclosed, automated, programmed, continuous and efficient integrated culture system, which greatly reduces the risk of contamination during the culture process. improve;

[0006] 3) The cell factories currently on the market can achieve the purpose of harvesting a large number of cells in one culture, but they take up a lot of space, and the cultivation process requires a large amount of culture medium, trypsin and other reagents. Taking the ten-layer cell factory as an example, the product height reaches 20cm. After filling the culture solution, the weight of the product increases significantly, and manual operation is very difficult. The whole process needs to be assisted by manipulators. The product is large in size and does not fit the laboratory incubator. It is necessary to purchase a special incubator, which will greatly increase equipment investment and consume a lot. Manpower, time, and space limit its widespread use

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