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Burkholderia and application thereof

A technology of Burkholderia and engineering bacteria, applied in application, bacteria, fungicides, etc., can solve problems such as pesticide residues, pathogenic bacteria resistance, environmental pollution, etc., and achieve good bacteriostatic effect.

Active Publication Date: 2019-01-18
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, chemical pesticides are mainly used to control them, but often due to environmental reasons such as rainfall, the control effect is not good, and it also brings many problems such as environmental pollution, pesticide residues, and pathogenic bacteria resistance.

Method used

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  • Burkholderia and application thereof
  • Burkholderia and application thereof
  • Burkholderia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] 1. Isolation and purification of strains

[0038] Randomly select 5 points in the rice planting area of ​​the Fuyang base of the China Rice Research Institute to dig rice roots, gently wash the floating soil on the root system with running water, and wash the soil closely attached to the root system by ultrasonic vibration. After gradient dilution of the above soil suspension, spread it on the LB culture dish, place the culture dish in a constant temperature incubator at 28°C, and pick colonies of different forms to streak and purify on fresh LB medium plates whenever the colonies grow. , and then pick a single colony and store it on a LB solid slope at 4°C for short-term activity determination. At the same time, a single colony was inoculated with LB liquid, and after overnight shaking culture at 28°C, the bacterial liquid was mixed with 40% sterilized glycerol in equal volumes and stored at -80°C for a long time.

[0039] Among the many isolated strains, a strain wit...

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PUM

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Abstract

The invention relates to the technical field of Burkholderia, in particular to a Burkholderia (Burkholderia), which is deposited in a Chinese typical culture collection center, and the collection number is CCTCC NO: M 2018703. It has bacteriostatic effect on sheath blight, rice blast and bakanae.

Description

technical field [0001] The invention relates to the technical field of Burkholderia. Background technique [0002] Fungal diseases seriously endanger global food security, and 70%-80% of plant diseases are caused by the infection of pathogenic fungi. Rice blast, sheath blight and bacterial blight are the three major diseases of rice. Other important diseases include rice false smut and bakanae disease. [0003] In recent years, with the accelerated renewal of rice varieties, frequent transportation, increased fertilization levels, and large-scale planting of rice, especially high-quality rice and hybrid rice have been infected more and more, affecting the proportion of middle and late rice in my country. At home and abroad, chemical pesticides are mainly used to control it, but the control effect is often poor due to environmental reasons such as rainfall, and it also brings many problems such as environmental pollution, pesticide residues, and pathogenic bacteria resistan...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 冯国忠吴丽娟严清陈国庆
Owner CHINA NAT RICE RES INST
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