A method and application of plag1 gene snp marker-assisted rapid detection of growth traits of cattle

A PLAG1 and detection method technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as the functional correlation between SNP and PLAG1 that have not been seen, and achieve low-cost effects

Active Publication Date: 2021-08-17
NORTHWEST A & F UNIV
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Problems solved by technology

Different genotypes of the known PLAG1 gene affect the transcription level of IGF2. PLAG1 binds to the third promoter of IGF2 and activates transcription to promote the transcription and expression of IGF2. IGF2 plays an important role in regulating the growth and development of muscle cells. See the research report on the functional correlation between SNP and PLAG1

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  • A method and application of plag1 gene snp marker-assisted rapid detection of growth traits of cattle
  • A method and application of plag1 gene snp marker-assisted rapid detection of growth traits of cattle
  • A method and application of plag1 gene snp marker-assisted rapid detection of growth traits of cattle

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the drawings and embodiments, and the embodiments are to explain the present invention rather than limit it.

[0024] In the present invention, primers are firstly designed according to the PLAG1 gene sequence published by NCBI, and the genomic DNAs of two cattle breeds are respectively used as templates to carry out PCR amplification, and the PCR products are mixed and sequenced. Then, sequence analysis and sequence comparison were carried out to screen out the SNP sites; secondly, the PCR-RFLP detection of polymorphic sites was carried out in the yellow cattle population to be tested; finally, according to the detected genotypes, population genetic statistical analysis and The association analysis of growth traits screened out the molecular markers closely related to the growth traits of cattle.

[0025] Cloning and SNP Screening of Partial DNA Sequence of PLAG1 Gene in Local Yellow Cattle Var...

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Abstract

The invention discloses a method for quickly detecting the growth traits of yellow cattle assisted by SNP markers of the PLAG1 gene and its application: the whole genome DNA of the yellow cattle to be tested is used as a template, and a partial fragment of the PLAG1 gene of the yellow cattle is amplified by PCR; Agarose gel electrophoresis, to realize the detection of the base polymorphism site at position 48308 of the cattle PLAG1 gene, which is C>T; correlation analysis confirmed that the TC and CC genotypes are closely related to the growth traits of cattle, and can be used as molecular genetic markers In molecular marker-assisted selective breeding of cattle, the establishment of cattle populations with excellent genetic resources can be accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for detecting the single nucleotide polymorphism (SNP) of the yellow cattle PLAG1 gene, a detection kit and an application in yellow cattle molecular breeding. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a type of genetic marker system proposed by Lander, a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology, which refers to the change of a single nucleotide in the genomic DNA sequence. The polymorphisms caused are mainly caused by the conversion or transversion of a single base. SNPs with transformational base variation accounted for about 2 / 3. Various numbers of SNPs may be found in or near any known or unknown gene. There are relatively few SNPs (cSNPs) in the coding region of the gene, because the variation rate in the exon only accounts for 1 / 5 of the surrounding sequence, but ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2521/301
Inventor 黄永震钟佳琳王健陈宏雷初朝党瑞华蓝贤勇郑立胡沈荣
Owner NORTHWEST A & F UNIV
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