Method for extracting DNA from plant genome and optimizing amplification system

Abstract

The invention discloses a method for extracting DNA from plant genome and optimizing an amplification system. The method comprises the following steps: taking a plant tissue sample which is 3-5mm longand 2-3 mm wide into a micro-pore plate; adding zirconia balls into the micro-pore plate; adding a DNA extracting solution into the micro-pore plate; and sealing the micro-pore plate, putting the sealed micro-pore plate onto a tissue grinding apparatus to be ground to form sample tissue liquid; taking the sample tissue liquid as a PCR reaction template to perform PCR amplification. The method canbe applied to quick extraction of DNA from various plant genomes, meets research of the plant molecular biology and the genetics, and rapid identification of genetically modified crops.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a method for quickly extracting plant genome DNA and optimizing an amplification system. Background technique [0002] There are two main methods of plant genome extraction commonly used in laboratories: SDS extraction and CTAB extraction. The extraction process of these two methods is cumbersome and time-consuming, and a variety of toxic and harmful organic reagents are frequently used in the extraction process, resulting in a large amount of waste liquid. [0003] At present, the principle of the kit method promoted on the market is to separate DNA according to the difference in molecular weight of nucleic acid or the specific adsorption of DNA by a specific membrane. Compared with the plant genome extraction method commonly used in the laboratory, this method is simplified. The operation process is shortened, but it is still not simple enough and expensive. It is not ...

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Problems solved by technology

The extraction process of these two methods is cumbersome and time-consuming, and a variety of toxic and harmful organic reagents are frequently used in the extraction process, resulting in a large amount of waste liquid

[0003] At present, the principle of the kit method promoted on the market is to separate DNA according to the difference in molecular weight of nucleic acid or the specific adsorption of DNA by a specific membrane. Compared with the plant genome extraction method commonly used in the laboratory, this method is simplified. The operation process is shortened, but it is still not simple enough and expensive. It is not suitable for large-scale genome extraction experiments such as population genetics, molecular marker-assisted breeding, and transgenic plant screening. It is suitable for a small amount of high-purity plant genomic DNA. extract

[0004] In addition, Chinese patents CN102839167A and CN105087550A respectively disclose a simple, rapid and high-throughput method for extracting plant genomic DNA. These two methods optimize the experimental process, but the operation process is complicated, and toxic and harmful organic reagents are used many times, and time-consuming too long

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