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Breeding method for black-endosperm rice with high anthocyanin content

A high anthocyanin and black technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problem of commercialization of transgenic black endosperm rice, and achieve the effect of guaranteeing the selection ratio

Active Publication Date: 2018-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, under the current background, the commercialization of the genetically modified black endosperm rice "Amethyst rice" is very difficult.

Method used

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  • Breeding method for black-endosperm rice with high anthocyanin content
  • Breeding method for black-endosperm rice with high anthocyanin content

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] 1. Breeding materials:

[0038] (1) Parent A is ZDR-hal-01. ZDR-hal-01 is a special-purpose rice jointly bred by Zhejiang University and Haining Kangrui Biotechnology Co., Ltd., and can be purchased from the Institute of Nucleus Agricultural Sciences, Zhejiang University. The fat content of the rice is as high as 2.3%, and the fat mainly exists in the form of starch-fat complex, the rice seed coat is normal light yellow, the endosperm is normal white, basically does not contain anthocyanin, and the content is extremely low.

[0039](2) Parent B is Heizhen of Zhejiang University. Zhejiang University Heizhen is a special black glutinous rice rice bred by the Institute of Nucleus Agricultural Sciences of Zhejiang University, which can be purchased from the institute. The seed coat of the rice is black, the endosperm is normal white, basically does not contain anthocyanin, has low fat content, and the fat mainly exists in the form of non-starch-fat complex.

[0040] 2. D...

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Abstract

The invention discloses a breeding method for black-endosperm rice with a high anthocyanin content. The breeding method includes the steps of taking rice rich in starch-fat complexes as a parent A, rice with black testa as a parent B, taking the parent B as a female parent, and hybridizing the parent B with the parent A to obtain an F1-generation seed; backcrossing the seed with the parent A to obtain a BC1F1 generation; sequentially planting BC1F1 to BC1F6 generations for self-crossing, and harvesting the black-endosperm rice with the high anthocyanin content according to screening indexes such as light yellow testa, black endosperm, the content of crude fat being higher than or equal to 2%, existence of 70% or more of the rice in the form of the starch-fat complexes, and the anthocyanincontent. The obtained rice has the advantages of light yellow testa, black endosperm, high content of the starch-fat complexes and high anthocyanin content, the anthocyanin is effectively expressed inedible endosperm portions, natural high-anthocyanin-content products such as black rice noodles can be developed without use of a food coloring agent, and accordingly, people's general requirements for natural, safe and healthy food are met.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding rice with black endosperm and high anthocyanin content. Background technique [0002] Anthocyanins, also known as anthocyanins, are a class of natural pigments that widely exist in plants in nature, and are widely used in food coloring, dyes, medicine, cosmetics, etc. Anthocyanins are phytonutrients belonging to bioflavonoids. They not only have strong free radical scavenging ability and anti-oxidation ability, but also have active molecules such as reducing enzyme activity and anti-mutation. They have important health care functions for human health. [0003] Colored special rice such as black rice, red rice and purple rice is rich in anthocyanins, which is the main material of traditional food supplements in my country. There are researches devoted to the breeding of colored special rice. [0004] The invention patent application with the applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 沈易李冰清张宁吴殿星
Owner ZHEJIANG UNIV
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