A kind of cultivation method of isolated microspore regeneration plant of rapeseed

Abstract

The invention belongs to the technical field of plant tissue culture, and discloses a method for cultivating rapeseed microspore regenerated plants in vitro. The method utilizes a certain concentration of colchicine solution to soak the regenerated plants cultivated by hydroponics with a plant height of 15-25 cm. Roots, and then continue hydroponics to regenerate plants and transplant them into fields. The regenerated plants after hydroponic cultivation are strong, with well-developed root system and strong root vitality. When the roots are soaked in colchicine solution, the root system can fully and evenly absorb the colchicine components, and the doubling effect is good. The cultivation method of the present invention does not need to dig seedlings and wash roots, and has no root system damage in the whole process, improves the survival rate of regenerated plants, is simple and easy to operate, easy to implement, low in cost, and convenient for large-scale production and application.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for cultivating rapeseed microspore regenerated plants in vitro. Background technique [0002] my country is a big oil consumption country in the world. The supply of domestic edible vegetable oil has been seriously insufficient for a long time, the import pressure remains high, and safety hazards are becoming increasingly prominent. Rapeseed oil is the largest source of domestic edible vegetable oil, accounting for more than 50% of the oil production of domestic oil crops. Therefore, the stable and healthy development of the rapeseed industry is an important guarantee for the safety of vegetable oil supply in my country. [0003] The high-yield and high-quality development of the rapeseed industry is inseparable from the support of excellent varieties. In the research and development of new rapeseed varieties, microspore culture technology plays a vital role,...

AI Technical Summary

Problems solved by technology

Among these three methods, the doubling rate of the colchicine root soaking method is the highest, but the traditional root soaking doubling method needs to dig out the haploid plants from the soil first, wash the root soil and use a certain concentration of colchicine solution Soak the roots for 2 to 6 hours, then rinse the roots with clean water and transplant them into the soil. The operation is cumbersome, especially for large-scale treatment, the workload of digging seedlings, washing roots, and transplanting is very large; secondly, the process of digging seedlings and transplanting In the middle, it is easy to damage the root system, which will have a very adverse effect on the growth of the plant.

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