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Standard breeding method for greater wax moth in laboratory

A breeding method and technology of C. mellonella, which is applied in the field of standardized breeding of experimental resources—Cellsella mellonella laboratory, can solve the problems of wide growth cycle range, inconsistent growth rate, low standardization degree of C. mellonella, and achieve an increase in annual growth rate. The effect of breeding batches, improving the pass rate, and improving the utilization rate

Inactive Publication Date: 2018-12-04
ZHENGZHOU IMMUNO BIOTECH
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Problems solved by technology

[0005] In order to overcome the deficiencies of the prior art and meet the needs of laboratory research, the present invention provides a standardized breeding method for the greater wax moth in the laboratory, which overcomes the low degree of standardization of the current culture of the greater wax moth, inconsistent growth rates, wide range of growth cycles, and difficult harvesting. The same batch of Melonella mellonella mature larvae ratio is low and other issues

Method used

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  • Standard breeding method for greater wax moth in laboratory
  • Standard breeding method for greater wax moth in laboratory
  • Standard breeding method for greater wax moth in laboratory

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Embodiment Construction

[0026] Below in combination with the process flow chart, the method of the present invention will be described in more detail.

[0027] like figure 1 Shown, the laboratory standardized breeding method of the greater wax moth of the present invention is:

[0028] The first step is to obtain the provenance of the wax moth

[0029] The source of the wax moth is the mature larvae of the wax moth obtained through purchase. The mature larvae are generally stored in artificial nests, and stored in a refrigerator at 14-16°C after purchase (which can reduce the pupation rate). The cycle can reach 3 months;

[0030] The second step, pupation and eclosion culture

[0031] The mature larvae taken out of the freezer are placed in the egg collection box of the wax moth (the authorized utility model patent "Egg Collection Box of the wax moth", patent number ZL 201720844413.7, publication number CN206963722U) can be used for cultivation (do not put the mature larvae The larvae are strippe...

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Abstract

The invention discloses a standard breeding method for greater wax moth in a laboratory. Aged larvae with a fixed number are placed in a greater wax moth egg collecting box for carrying out pupation culture, and after adult emergence is completed, adults freely mate and lay eggs in the greater wax moth egg collecting box; egg pieces are collected for preservation at a low temperature; the egg pieces with a fixed mass are placed in larva breeding boxes for carrying out culture to a certain stage, wherein the mass of an incubation medium in each larva breeding box is constant; larvae with uniform sizes are screened and are put into the larva breeding boxes for carrying out culture, wherein the mass of a culture medium and the quantity of the larvae in each breeding box are constant, so thatthe growth rate of the larvae is basically consistent, the time for becoming aged larvae is basically consistent, the harvesting efficiency of the aged larvae and the feed utilization ratio are improved, and the harvested greater wax moth can be better applied to the fields of nematology study, pesticide development, bait processing and the like.

Description

technical field [0001] The invention relates to the technical field of insect breeding for experiments, in particular to an experimental resource---a laboratory standardized breeding method of the wax moth. Background technique [0002] The wax moth (Galleria mellonella) belongs to the insects of Lepidoptera, Mothidae, and Mellonella subfamily. It is a complete metamorphosis insect, and its life cycle includes four developmental stages: egg, larva, pupa and adult. The larvae of the wax moth feed on the comb, which is more harmful to the beekeeping industry. In addition, it can also harm fur and plants. There are two types of wax moths, the greater wax moth and the lesser wax moth. Due to its short growth cycle, abundant food sources, and easy mass reproduction, it has been gradually used in medicine, pharmacy, agronomy and other fields in recent years, such as nematology research, pesticide development, bait processing, etc. Based on its high economic value and scientific r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90
CPCA01K67/033A23K50/90
Inventor 王萍赵巧辉李桂林付光宇吴学炜
Owner ZHENGZHOU IMMUNO BIOTECH
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