Liquid phase chip detection method for porcine parvovirus

Abstract

The invention discloses a liquid phase chip detection method for porcine parvovirus. A purpose of the present invention is to provide a liquid phase chip detection method of PPV antibody, wherein themethod has advantages of high specificity, high sensitivity, low detection cost and simple operation. The technical scheme comprises: 1) coating with antigen; and 2) diluting a serum sample 2 times, diluting the coated microspheres to achieve a concentration of 50/[mu]L with PBS-TBN, resuspending the microspheres, adding 50 [mu]L of the diluted microspheres to each well of a 96-well ELISA plate, adding the serum sample, incubating for 120 min, separating the microspheres, adding 100 [mu]L of a PBS-TBN washing liquid to each well, removing the washing solution, removing the reaction plate fromthe magnetic separator, adding 100 [mu]L of biotin-labeled sheep anti-porcine secondary antibody, incubating, separating the microspheres, removing the reaction solution and the washing liquid, adding100 [mu]L of phycoerythrin-labeled streptavidin to each well, incubating for 30 min, separating the microspheres, removing the washing liquid, adding 100 [mu]L of PBS-TBN, placing the reaction plateon an oscillator, oscillating for 1 min, loading the sample, reading the fluorescence median by using a luminex system, and calculating the ratio of the positive MFI value to the negative MFI value.

Description

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AI Technical Summary

Benefits of technology

This technology allows us to detect very small amounts (<10^g) of an unknown substance that may be harmful or even fatal if it were introduced into our body during pregnancy. It also makes this process faster than previous methods like ELISA because we only needed one type of molecule per sample instead of two types at once.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to prevent or treat diseases associated with PPRSV infections such as abortion syndrome due to their ability to cause symptoms like abnormal growth rates on young horses during breeding cycles.

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