Paternity identification method based on fetal free DNA in peripheral blood of pregnant women

Abstract

The invention relates to the technical field of biogenetics, in particular to a paternity identification method based on fetal free DNA in peripheral blood of pregnant women. The detection sample usedin the paternity identification method based on fetal free DNA in peripheral blood of pregnant women is easy to obtain, and effectively avoids unnecessary medical risks caused to pregnant women or fetuses; meanwhile, the exclusion index X1 provided by the invention and the established evidence value evaluation model of the paternity identification (formula IV, formula V, formula VI, etc ) not only can effectively avoid misjudgment, but also have higher identification efficiency. In addition, the paternity identification method is simple to operate and high in analysis efficiency; therefore, the detection result obtained by performing the paternity identification method based on fetal free DNA in peripheral blood of pregnant women can be further used as an effective legal evidence, so thatthe paternity identification method based on fetal free DNA in peripheral blood of pregnant women can be widely used in application fields such as judicial paternity identification.

Description

technical field [0001] The invention relates to the technical field of biological genetics, in particular to a paternity identification method based on fetal free DNA in the peripheral blood of pregnant women. Background technique [0002] According to the statistics of American scholars, about 5% of raped women will become pregnant due to rape. In such cases, it is often not possible to determine whether the biological father of the fetus is the rapist or the partner of the victim until a paternity test is performed. [0003] In the prior art, the paternity test before delivery of the fetus mainly includes two schemes: [0004] The first option is: after the fetus reaches a certain gestational age (at least 13-14 weeks), prenatal samples are obtained through amniocentesis, or chorionic villus biopsy, etc., and the traditional method based on short tandem repeat (STR) genetic Marked forensic paternity test kits are used to carry out paternity tests on victims, rape suspect...

AI Technical Summary

Problems solved by technology

Therefore, when using NGS technology to detect a low proportion of DNA in a mixed sample, it depends on the depth of sequencing coverage. For example, when detecting a low proportion of 1% DNA, the sequencing depth of NGS should be at least 200 times; When it is lower, it may be due to insufficient sequencing depth that the paternal allele should be detected but not detected, thus forming a "false" homozygous result of the fetal maternal allele at such a site, while multiple Such sites will inevitably lead to misjudgment of the final paternity test results

[0009] In addition to the above-mentioned technical defects, the existing NGS technical scheme itself lacks the research on the statistical model of possible genetic evidence value indicators; parental judgments are made only based on the number of so-called incompatible loci based on experience. On the one hand, there are misunderstandings. The possibility of judging, the more important aspect is that due to the lack of a scientific evidence value evaluation system, its test results are also difficult to be used as an effective legal evidence

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