A liquid droplet preparation method and device based on active control of oscillatory flow

Abstract

The present invention proposes a liquid droplet preparation method based on the active control of oscillatory flow. First, the liquid droplets of the first discrete phase at intervals are prepared, and then the liquid droplets of the second discrete phase at intervals are prepared synchronously. Finally, the liquid droplets of the first discrete phase are prepared The droplet and the second discrete phase droplet are arranged and output through different length output pipes; the present invention also discloses a liquid droplet preparation device based on the active control of oscillating flow, including M T-shaped channels and a negative flow resistance oscillator; The negative flow resistance oscillator is used to oscillate the continuous phase liquid into a sine wave flow, the first liquid inlet pipe of the T-shaped channel is used as the inflow channel of the first discrete phase liquid; the second inlet pipe of the T-shaped channel The liquid pipe serves as the inflow channel of the continuous phase liquid; the liquid outlet pipes of the M T-shaped channels are jointly connected with the output pipe; the present invention improves the reliability of the preparation of high-viscosity liquid droplets, and simplifies the generation frequency and volume in the liquid droplet preparation process The operation steps simultaneously solve the simultaneous generation of multi-droplets of different properties.

Description

technical field [0001] The invention belongs to the field of microfluidic devices, in particular to a droplet preparation method and device based on active control of oscillating flow. Background technique [0002] The droplet generation methods can be mainly divided into two categories: passive and active. The passive micro-droplet preparation technology involves many variables. The volume and frequency of micro-droplet formation are not only related to the flow rate of the fluid and the size of the channel structure, but also It is related to the properties of the fluid (viscosity, interfacial tension), external environment and other parameters. Therefore, controlling the volume and frequency of droplets through passive droplet preparation requires a complex debugging process, which also leads to the difficulty of micro-droplet preparation. Flexibility is greatly reduced. Although the active droplet preparation technology can solve the technical problems of poor volume an...

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Problems solved by technology

[0002] The droplet generation methods can be mainly divided into two categories: passive and active. The passive micro-droplet preparation technology involves many variables. The volume and frequency of micro-droplet formation are not only related to the flow rate of the fluid and the size of the channel structure, but also It is related to the properties of the fluid (viscosity, interfacial tension), external environment and other parameters. Therefore, controlling the volume and frequency of droplets through passive droplet preparation requires a complex debugging process, which also leads to the difficulty of micro-droplet preparation. Much less flexibility

Although the active droplet preparation technology can solve the technical problems of poor volume and frequency controllability in the traditional passive droplet preparation technology, the active droplet preparation technology requires additional equipment to interfere with the droplet formation.

Due to the existence of external equipment, the structure of the active micro-droplet chip is often more complex, which affects the reliability, and the volume is larger, the cost is higher, and the portability is poor.

In addition, the external field force applied in the active control often acts on the reagent, which is easy to have adverse effects or damage to the reagent and affect the experimental results.

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