A compound composition capable of reducing the content of cardiac creatine kinase isozyme and its application

A technology of creatine kinase and composition, which is applied to a compound composition with the function of reducing the content of cardiac creatine kinase isoenzyme and its application field, can solve problems such as poisoning and myocardial damage, and achieves reducing myocardial damage and recovering myocardial damage. , the effect of restoring the heart damage

Active Publication Date: 2020-02-21
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some alcohol additives contain toxic substances such as cobalt and lead, and long-term drinking can cause poisoning or myocardial damage.

Method used

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  • A compound composition capable of reducing the content of cardiac creatine kinase isozyme and its application
  • A compound composition capable of reducing the content of cardiac creatine kinase isozyme and its application
  • A compound composition capable of reducing the content of cardiac creatine kinase isozyme and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Materials:

[0040] Reagents: ursolic acid; dihydromyricetin; curcumin; absolute ethanol; LDH detection kit, CKMB detection kit.

[0041] 2. Cell lines and medium:

[0042] H9C2 cardiomyocytes were purchased from ATCC. FBS (biological industries).

[0043] DMEM high-glucose culture medium (Gino Biomedical Technology Co., Ltd.), absolute ethanol.

[0044] 3. Experimental method

[0045] This experiment is divided into 6 groups, control group (UT), alcohol model group (alcohol group), + alcohol group (ursolic acid), dihydromyricetin + alcohol group (dihydromyricetin), curcumin + alcohol group (curcumin), compound + alcohol group (compound).

[0046] Inoculate H9C2 cells into a 24-well plate containing 10% FBS in DMEM high-glucose medium, 1*10 per well 5After culturing at 37°C for 24 hours, the supernatant was discarded, and 1 mL of 10% FBS+DMEM high-glucose culture solution containing different drugs was added to each group of culture wells. The final concentrat...

Embodiment 2

[0051] Change grouping and each drug dosage into 6 groups in embodiment 1, control group (UT), alcohol model group (alcohol), dihydromyricetin+ursolic acid+alcohol group (dihydromyricetin 15 μ M+ ursolic acid 40 μ M) , Dihydromyricetin + curcumin + alcohol group (dihydromyricetin 15μM + curcumin 15μM), curcumin + ursolic acid + alcohol group (curcumin 15μM + ursolic acid 40μM), dihydromyricetin + ursolic acid + Curcumin + alcohol group (dihydromyricetin 15 μM + ursolic acid 15 μM + curcumin 15 μM). Other operations are the same as embodiment 1, the results are shown in figure 2 shown.

[0052] figure 2 It shows that the joint action of the three compounds of the compound can effectively reduce the lactate dehydrogenase (LDH) released by ethanol-induced cell damage figure 2 Middle A) and creatine kinase isoenzyme CKMB ( figure 2 In B), the effect is more significant than that of the two monomeric compounds. Therefore, this compound can effectively protect cardiomyocyte...

Embodiment 3

[0054] Change grouping and drug concentration in embodiment 1 to: control group (UT), alcohol model group (alcohol), berberine+dihydromyricetin+ursolic acid+alcohol group (berberine 20 μ M+ dihydromyricetin 15 μ M+ bearberry acid 40 μM), berberine + dihydromyricetin + curcumin + alcohol group (berberine 20 μM + dihydromyricetin 15 μM + curcumin 15 μM), berberine + curcumin + ursolic acid + alcohol group (berberine 20 μM + curcumin 15 μM+ ursolic acid 40 μM), dihydromyricetin + ursolic acid + curcumin + alcohol group (dihydromyricetin 15 μM + ursolic acid 40 μM + curcumin 15 μM). Other operations are the same as embodiment 1, the results are shown in image 3 shown.

[0055] image 3 It shows that berberine has the effect of protecting myocardial injury in the current report, so this experiment compares the effect of monomer compound components in the replacement compound on the protection of myocardial cells, and the results show that berberine can not replace the compounds ...

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Abstract

The invention discloses a compound composition with the function of decreasing the cardiac CK-MB content and application thereof. According to the compound composition, the plasma CK-MB content in analcoholic cardiomyopathy model is decreased by 46.86%, the cardiac ejection capability in alcoholic cardiomyopathy is restored, the myocardial hypertrophy is alleviated, and the myocardial damage caused by the alcoholic cardiomyopathy model is remarkably reduced; at present, clinical manifestation of the alcoholic cardiomyopathy is often accompanied by cardiac dilatation and congestive heart failure, mainly left ventricular failure; and the compound composition can effectively restore and increase the left ventricle posterior wall thickness and enable a patient to recover from myocardial damage, so that the patient can be effectively recovered from the cardiac damage situation caused by an alcoholic diet model.

Description

[0001] (1) Technical field [0002] The invention relates to a composition of effective fractions of traditional Chinese medicines capable of treating alcoholic cardiomyopathy, that is, the application of the compound composition in drugs for preventing or treating alcoholic cardiomyopathy. [0003] (2) Background technology [0004] Alcohol cardiomyopathy (alcoholic cardiomyopathy, ACM) is a growing disease in the cardiovascular field. Due to long-term drinking, it leads to myocardial degeneration, which is a kind of cardiomyopathy manifested as heart enlargement and cardiac insufficiency. It is closely related to alcohol intake, and has the hemodynamic changes, symptoms, signs and imaging findings of typical dilated cardiomyopathy. Alcoholic cardiomyopathy is the result of direct toxicity of ethanol and its metabolite acetaldehyde to the myocardium. The direct toxic effect of alcohol on cardiomyocytes is mainly manifested in the following aspects: ① damage the integrity of m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K31/56A61K31/12A61P9/00A61P9/04
CPCA61K31/12A61K31/352A61K31/56A61P9/00A61P9/04A61K2300/00
Inventor 窦晓兵杜珊珊丁秦超丁滨李松涛
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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